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TitleProtocol for high-yield bacterial expression and purification of the voltage-dependent anion channel 1 for high-throughput biophysical assays.
Journal, issue, pagesSTAR Protoc, Vol. 6, Page 103557-103557, Year 2025
Publish dateSep 3, 2024 (structure data deposition date)
AuthorsConti Nibali, S. / Magri, A. / Messina, A. / Wagner, A. / Duman, R. / De Pinto, V. / Turato, C. / Arrigoni, C. / Lolicato, M.
External linksSTAR Protoc / PubMed:39799574
MethodsX-ray diffraction
Resolution2.4 Å
Structure data

PDB-9gng:
mouse VDAC1 in complex with MPD
Method: X-RAY DIFFRACTION / Resolution: 2.4 Å

Chemicals

ChemComp-MPD:
(4S)-2-METHYL-2,4-PENTANEDIOL / precipitant*YM

ChemComp-PX4:
1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE / DMPC, phospholipid*YM

ChemComp-HOH:
WATER

Source
  • mus musculus (house mouse)
KeywordsMEMBRANE PROTEIN / ion channel / VA binding site / NADH

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