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TitleCryo-EM structure of an activated GPR4-Gs signaling complex.
Journal, issue, pagesNat Commun, Vol. 16, Issue 1, Page 605, Year 2025
Publish dateJan 11, 2025
AuthorsYitong Ma / Yijie Wang / Mengyuan Tang / Yuan Weng / Ying Chen / Yueming Xu / Shuxiao An / Yiran Wu / Suwen Zhao / Huanhuan Xu / Dali Li / Mingyao Liu / Weiqiang Lu / Heng Ru / Gaojie Song /
PubMed AbstractG protein-coupled receptor 4 (GPR4) belongs to the subfamily of proton-sensing GPCRs (psGPCRs), which detect pH changes in extracellular environment and regulate diverse physiological responses. GPR4 ...G protein-coupled receptor 4 (GPR4) belongs to the subfamily of proton-sensing GPCRs (psGPCRs), which detect pH changes in extracellular environment and regulate diverse physiological responses. GPR4 was found to be overactivated in acidic tumor microenvironment as well as inflammation sites, with a triad of acidic residues within the transmembrane domain identified as crucial for proton sensing. However, the 3D structure remains unknown, and the roles of other conserved residues within psGPCRs are not well understood. Here we report cryo-electron microscopy (cryo-EM) structures of active zebrafish GPR4 at both pH 6.5 and 8.5, each highlighting a distribution of histidine and acidic residues at the extracellular region. Cell-based assays show that these ionizable residues moderately influence the proton-sensing capacity of zebrafish GPR4, compared to the more significant effects of the triad residues. Furthermore, we reveal a cluster of aromatic residues within the orthosteric pocket that may propagate the signaling to the intercellular region via repacking the aromatic patch at the central region. This study provides a framework for future signaling and functional investigation of psGPCRs.
External linksNat Commun / PubMed:39799123 / PubMed Central
MethodsEM (single particle)
Resolution3.05 - 3.3 Å
Structure data

60072

8zfz

EMDB-60072, PDB-8zfz:
cryo-EM structure of Gs-coupled zebrafish GPR4 at pH 6.5
Method: EM (single particle) / Resolution: 3.3 Å

61104

9j31

EMDB-61104, PDB-9j31:
cryo-EM structure of zebrafish GPR4-Gs complex at pH 8.5
Method: EM (single particle) / Resolution: 3.05 Å

Source
  • danio rerio (zebrafish)
  • homo sapiens (human)
  • mus musculus (house mouse)
  • synthetic construct (others)
  • escherichia coli (E. coli)
KeywordsSIGNALING PROTEIN / GPCR / membrane protein. / SIGNALING PROTEIN/IMMUNE SYSTEM / proton-sensing / G protein / SIGNALING PROTEIN-IMMUNE SYSTEM complex

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