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TitleComparative Analysis of Symmetry Parameters in the E2 Inner Core of the Pyruvate Dehydrogenase Complex.
Journal, issue, pagesInt J Mol Sci, Vol. 25, Issue 24, Year 2024
Publish dateDec 23, 2024
AuthorsHan-Ul Kim / Myeong Seon Jeong / Mi Young An / Yoon Ho Park / Sun Hee Park / Sang J Chung / Yoon-Sun Yi / Sangmi Jun / Young Kwan Kim / Hyun Suk Jung /
PubMed AbstractRecent advances in cryo-electron microscopy (cryo-EM) have facilitated the high-resolution structural determination of macromolecular complexes in their native states, providing valuable insights ...Recent advances in cryo-electron microscopy (cryo-EM) have facilitated the high-resolution structural determination of macromolecular complexes in their native states, providing valuable insights into their dynamic behaviors. However, insufficient understanding or experience with the cryo-EM image processing parameters can result in the loss of biological meaning. In this paper, we investigate the dihydrolipoyl acetyltransferase (E2) inner core complex of the pyruvate dehydrogenase complex (PDC) and reconstruct the 3D maps using five different symmetry parameters. The results demonstrate that the reconstructions yield structurally identical 3D models even at a near-atomic structure. This finding underscores a crucial message for researchers engaging in single-particle analysis (SPA) with relatively user-friendly and convenient image processing software. This approach helps reduce the risk of missing critical biological details, such as the dynamic properties of macromolecules.
External linksInt J Mol Sci / PubMed:39769492 / PubMed Central
MethodsEM (single particle)
Resolution4.2 - 4.94 Å
Structure data

EMDB-38333: Cryo-EM map of the E2 inner core component of pyruvate dehydrogenase complex
Method: EM (single particle) / Resolution: 4.94 Å

EMDB-38334: Cryo-EM map of the E2 inner core component of pyruvate dehydrogenase complex
Method: EM (single particle) / Resolution: 4.76 Å

EMDB-38335: Cryo-EM map of the E2 inner core component of pyruvate dehydrogenase complex
Method: EM (single particle) / Resolution: 4.79 Å

EMDB-38336: Cryo-EM map of the E2 inner core component of pyruvate dehydrogenase complex
Method: EM (single particle) / Resolution: 4.2 Å

EMDB-38340: Cryo-EM map of the E2 inner core component of pyruvate dehydrogenase complex
Method: EM (single particle) / Resolution: 4.36 Å

Source
  • Homo sapiens (human)

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