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TitleStructural Basis of High-Precision Protein Ligation and Its Application.
Journal, issue, pagesJ. Am. Chem. Soc., Vol. 147, Page 1604-1611, Year 2025
Publish dateJun 22, 2023 (structure data deposition date)
AuthorsChong, K.H.C. / Liu, L. / Chua, R. / Chai, Y.T. / Lu, Z. / Liu, R. / Tan, E.Y.J. / Dong, J. / Khoh, Y.H. / Lin, J. ...Chong, K.H.C. / Liu, L. / Chua, R. / Chai, Y.T. / Lu, Z. / Liu, R. / Tan, E.Y.J. / Dong, J. / Khoh, Y.H. / Lin, J. / Zhong, F.L. / Lescar, J. / Zheng, P. / Wu, B.
External linksJ. Am. Chem. Soc. / PubMed:39745918
MethodsX-ray diffraction
Resolution1.98 - 3.4 Å
Structure data

PDB-8jtu:
Connectase T1A mutant from Methanocaldococcus mazei
Method: X-RAY DIFFRACTION / Resolution: 3.4 Å

PDB-8wkd:
Connectase T1A C192S mutant from Methanocaldococcus mazei with peptide substrate
Method: X-RAY DIFFRACTION / Resolution: 1.98 Å

Chemicals

ChemComp-HOH:
WATER

Source
  • methanosarcina mazei (archaea)
KeywordsLIGASE / methanogenic archaea / proteasome / protein ligation / sortase / transpeptidase / methanogenic archea

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