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-Structure paper
| タイトル | Molecular architectures of centrosomes in C. elegans embryos visualized by cryo-electron tomography. |
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| ジャーナル・号・ページ | Dev Cell, Vol. 60, Issue 6, Page 885-900.e5, Year 2025 |
| 掲載日 | 2025年3月24日 |
著者 | Fergus Tollervey / Manolo U Rios / Evgenia Zagoriy / Jeffrey B Woodruff / Julia Mahamid / ![]() |
| PubMed 要旨 | Centrosomes organize microtubules that are essential for mitotic divisions in animal cells. They consist of centrioles surrounded by pericentriolar material (PCM). Questions related to mechanisms of ...Centrosomes organize microtubules that are essential for mitotic divisions in animal cells. They consist of centrioles surrounded by pericentriolar material (PCM). Questions related to mechanisms of centriole assembly, PCM organization, and spindle microtubule formation remain unanswered, partly due to limited availability of molecular-resolution structural data inside cells. Here, we use cryo-electron tomography to visualize centrosomes across the cell cycle in cells isolated from C. elegans embryos. We describe a pseudo-timeline of centriole assembly and identify distinct structural features in both mother and daughter centrioles. We find that centrioles and PCM microtubules differ in protofilament number (13 versus 11), which could be explained by atypical γ-tubulin ring complexes with 11-fold symmetry identified at the minus ends of short PCM microtubule segments. We further characterize a porous and disordered network that forms the interconnected PCM. Thus, our work builds a three-dimensional structural atlas that helps explain how centrosomes assemble, grow, and achieve function. |
リンク | Dev Cell / PubMed:39721584 / PubMed Central |
| 手法 | EM (サブトモグラム平均) / EM (トモグラフィー) |
| 解像度 | 26.0 - 44.0 Å |
| 構造データ | ![]() EMDB-19778: in situ subtomogram average of C. elegans microtubules in mitotic centrosomes ![]() EMDB-19779: in-situ subtomogram average of C. elegans centrioles in centrosomes ![]() EMDB-19780: in situ subtomogram average of C. elegans gamma-tubulin ring complexes in mitotic centrosomes ![]() EMDB-19781: Cryo-ET of a mitotic centrosome in an embryonic C. elegans cell |
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