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TitleThe structural and functional analysis of mycobacteria cysteine desulfurase-loaded encapsulin.
Journal, issue, pagesCommun Biol, Vol. 7, Issue 1, Page 1656, Year 2024
Publish dateDec 19, 2024
AuthorsYanting Tang / Yanyan Liu / Mingjing Zhang / Weiqi Lan / Mengyuan Ma / Cheng Chen / Saibin Wu / Rong Chen / Yiran Yan / Lu Feng / Ying Li / Luke W Guddat / Yan Gao / Xiang Liu / Zihe Rao /
PubMed AbstractEncapsulin nanocompartments loaded with dedicated cargo proteins via unique targeting peptides, play a key role in stress resistance, iron storage and natural product biosynthesis. Mmp1 and cysteine ...Encapsulin nanocompartments loaded with dedicated cargo proteins via unique targeting peptides, play a key role in stress resistance, iron storage and natural product biosynthesis. Mmp1 and cysteine desulfurase (Enc-CD) have been identified as the most abundant representatives of family 2 encapsulin systems. However, the molecular assembly, catalytic mechanism, and physiological functions of the Mmp1 encapsulin system have not been studied in detail. Here we isolate and characterize an Enc-CD-loaded Mmp1 encapsulin system from Mycobacterium smegmatis mc155. The cryo-EM structure of the Mmp1 encapsulin and the crystal structure of the naked cargo Enc-CD have been determined. The structure shows that the Mmp1 protomer assembles two conformation models, the icosahedron (T = 1) and homodecamer, with the resolution of 2.60 Å and 2.69 Å. The Enc-CD at 2.10 Å resolution is dimeric and loaded into the Mmp1 (T = 1) encapsulin through the N-terminal long disordered region. Mmp1 encapsulin protects Enc-CD against oxidation as well as to maintain structural stability. These studies provide new insights into the mechanism by which Enc-CD-loaded encapsulin stores sulfur and provides a framework for discovery of new anti-mycobacterial therapeutics.
External linksCommun Biol / PubMed:39702509 / PubMed Central
MethodsEM (single particle)
Resolution2.6 - 3.08 Å
Structure data

37691

8wol

EMDB-37691, PDB-8wol:
Cryo-EM structure of the Mmp1 encapasulin from Mycobacterium smegmatis
Method: EM (single particle) / Resolution: 2.6 Å

37693

8won

EMDB-37693, PDB-8won:
Cryo-EM structure of the 10-subunits Mmp1 complex from Mycobacterium smegmatis
Method: EM (single particle) / Resolution: 2.69 Å

EMDB-60569: cryo-EM map of encapsulin Mmp1 with SufS
Method: EM (single particle) / Resolution: 3.08 Å

Source
  • mycolicibacterium smegmatis (bacteria)
KeywordsSTRUCTURAL PROTEIN / Mmp1 encapasulin / 10-subunits Mmp1 complex

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