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Structure paper

TitleStructures of a DNA Polymerase Caught while Incorporating Responsive Dual-Functional Nucleotide Probes.
Journal, issue, pagesAngew. Chem. Int. Ed. Engl., Vol. 64, Page e202414319-e202414319, Year 2025
Publish dateJun 5, 2024 (structure data deposition date)
AuthorsGhosh, P. / Betz, K. / Gutfreund, C. / Pal, A. / Marx, A. / Srivatsan, S.G.
External linksAngew. Chem. Int. Ed. Engl. / PubMed:39428682
MethodsX-ray diffraction
Resolution2.1 - 2.59 Å
Structure data

PDB-9fm3:
KlenTaq DNA polymerase in a ternary complex with primer/template and a selenophene-modified dUTP (SedUTP)
Method: X-RAY DIFFRACTION / Resolution: 2.59 Å

PDB-9fmf:
KlenTaq DNA polymerase in a ternary complex with primer/template and a fluorobenzofuran-modified dUTP (FBFdUTP)
Method: X-RAY DIFFRACTION / Resolution: 2.1 Å

Chemicals

PDB-1idz:
STRUCTURE OF MYB TRANSFORMING PROTEIN, NMR, 20 STRUCTURES

ChemComp-MG:
Unknown entry

ChemComp-DOC:
2',3'-DIDEOXYCYTIDINE-5'-MONOPHOSPHATE

ChemComp-HOH:
WATER

PDB-1idw:
STRUCTURE OF THE HYBRID RNA/DNA R-GCUUCGGC-D[CL]U IN PRESENCE OF RH(NH3)6+++

ChemComp-GOL:
GLYCEROL

Source
  • thermus aquaticus (bacteria)
  • synthetic construct (others)
KeywordsTRANSFERASE / DNA Polymerase / Selenophene / Fluorescent nucleotide probe / KlenTaq / Fluorobenzofuran

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