EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Broadly therapeutic antibody provides cross-serotype protection against enteroviruses via Fc effector functions and by mimicking SCARB2.
ジャーナル・号・ページ	Nat Microbiol, Vol. 9, Issue 11, Page 2939-2953, Year 2024
掲載日	2024年10月18日
著者	Rui Zhu / Yuanyuan Wu / Yang Huang / Yanan Jiang / Yichao Jiang / Dongqing Zhang / Hui Sun / Zhenhong Zhou / Lizhi Zhou / Shihan Weng / Hao Chen / Xiaoqing Chen / Wenjing Ning / Yuxiang Zou / Maozhou He / Hongwei Yang / Weixi Deng / Yu Li / Zhenqin Chen / Xiangzhong Ye / Jinle Han / Zhichao Yin / Huan Zhao / Che Liu / Yuqiong Que / Mujin Fang / Hai Yu / Jun Zhang / Wenxin Luo / Shaowei Li / Qingbing Zheng / Longfa Xu / Ningshao Xia / Tong Cheng /
PubMed 要旨	Enteroviruses contain multiple serotypes and can cause severe neurological complications. The intricate life cycle of enteroviruses involving dynamic virus-receptor interaction hampers the ...Enteroviruses contain multiple serotypes and can cause severe neurological complications. The intricate life cycle of enteroviruses involving dynamic virus-receptor interaction hampers the development of broad therapeutics and vaccines. Here, using function-based screening, we identify a broadly therapeutic antibody h1A6.2 that potently protects mice in lethal models of infection with both enterovirus A71 and coxsackievirus A16 through multiple mechanisms, including inhibition of the virion-SCARB2 interactions and monocyte/macrophage-dependent Fc effector functions. h1A6.2 mitigates inflammation and improves intramuscular mechanics, which are associated with diminished innate immune signalling and preserved tissue repair. Moreover, cryogenic electron microscopy structures delineate an adaptive binding of h1A6.2 to the flexible and dynamic nature of the VP2 EF loop with a binding angle mimicking the SCARB2 receptor. The coordinated binding mode results in efficient binding of h1A6.2 to all viral particle types and facilitates broad neutralization of enterovirus, therefore informing a promising target for the structure-guided design of pan-enterovirus vaccine.
リンク	Nat Microbiol / PubMed:39424982
手法	EM (単粒子)
解像度	2.89 - 3.82 Å
構造データ	38165 8x95 EMDB-38165, PDB-8x95: Cryo-EM structure of enterovirus A71 mature virion in complex with Fab h1A6.2 手法: EM (単粒子) / 解像度: 3.52 Å 38166 8x96 EMDB-38166, PDB-8x96: Cryo-EM structure of enterovirus A71 A-particle in complex with Fab h1A6.2 手法: EM (単粒子) / 解像度: 2.89 Å 38167 8x97 EMDB-38167, PDB-8x97: Cryo-EM structure of enterovirus A71 empty particle in complex with Fab h1A6.2 手法: EM (単粒子) / 解像度: 2.98 Å 38168 8x98 EMDB-38168, PDB-8x98: Cryo-EM structure of coxsackievirus A16 mature virion in complex with Fab h1A6.2 手法: EM (単粒子) / 解像度: 3.69 Å 38169 8x99 EMDB-38169, PDB-8x99: Cryo-EM structure of coxsackievirus A16 A-particle in complex with Fab h1A6.2 手法: EM (単粒子) / 解像度: 3.38 Å 38170 8x9a EMDB-38170, PDB-8x9a: Cryo-EM structure of coxsackievirus A16 empty particle in complex with Fab h1A6.2 手法: EM (単粒子) / 解像度: 3.36 Å 38171 8x9b EMDB-38171, PDB-8x9b: Cryo-EM structure of coxsackievirus A16 empty particle in complex with Fab h1A6.2 (local refinement) 手法: EM (単粒子) / 解像度: 3.82 Å 39570 8ytb EMDB-39570, PDB-8ytb: Cryo-EM structure of enterovirus A71 empty particle 手法: EM (単粒子) / 解像度: 2.96 Å 39572 8ytj EMDB-39572, PDB-8ytj: Cryo-EM structure of enterovirus A71 mature virion 手法: EM (単粒子) / 解像度: 3.07 Å
化合物	ChemComp-SPH: SPHINGOSINE
由来	Mus (ネズミ) enterovirus a71 (エンテロウイルス) coxsackievirus a16 (コクサッキーウイルス) mus musculus (ハツカネズミ)
キーワード	VIRUS / ENTEROVIRUS A71 / Cryo-EM STRUCTURE / Cryo-EM / coxsackievirus A16 / VIRAL PROTEIN