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TitleA Broad Spectrum Lasso Peptide Antibiotic Targeting the Bacterial Ribosome.
Journal, issue, pagesRes Sq, Year 2024
Publish dateAug 29, 2024 (structure data deposition date)
AuthorsWright, G. / Jangra, M. / Travin, D. / Aleksandrova, E. / Kaur, M. / Darwish, L. / Koteva, K. / Klepacki, D. / Wang, W. / Tiffany, M. ...Wright, G. / Jangra, M. / Travin, D. / Aleksandrova, E. / Kaur, M. / Darwish, L. / Koteva, K. / Klepacki, D. / Wang, W. / Tiffany, M. / Sokaribo, A. / Coombes, B. / Vazquez-Laslop, N. / Polikanov, Y. / Mankin, A.
External linksRes Sq / PubMed:39372947
MethodsX-ray diffraction
Resolution2.5 - 2.6 Å
Structure data

PDB-9dfc:
Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with lasso peptide lariocidin, mRNA, aminoacylated A-site Phe-tRNAphe, aminoacylated P-site fMet-tRNAmet, and deacylated E-site tRNAphe at 2.50A resolution
Method: X-RAY DIFFRACTION / Resolution: 2.5 Å

PDB-9dfd:
Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with lasso peptide lariocidin B, mRNA, aminoacylated A-site Phe-tRNAphe, aminoacylated P-site fMet-tRNAmet, and deacylated E-site tRNAphe at 2.60A resolution
Method: X-RAY DIFFRACTION / Resolution: 2.6 Å

PDB-9dfe:
Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with lasso peptide lariocidin and protein Y at 2.60A resolution
Method: X-RAY DIFFRACTION / Resolution: 2.6 Å

Chemicals

ChemComp-MG:
Unknown entry

ChemComp-K:
Unknown entry

ChemComp-ZN:
Unknown entry

ChemComp-SF4:
IRON/SULFUR CLUSTER

ChemComp-HOH:
WATER

ChemComp-ARG:
ARGININE

ChemComp-MPD:
(4S)-2-METHYL-2,4-PENTANEDIOL / precipitant*YM

Source
  • escherichia coli (E. coli)
  • paenibacillus (bacteria)
  • thermus thermophilus hb8 (bacteria)
  • escherichia phage t4 (virus)
  • escherichia coli k-12 (bacteria)
KeywordsRIBOSOME / Lasso peptide; RiPP; ribosome; antibiotic; tRNA; decoding; translocation; inhibitor; drug resistance

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