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TitleFilament formation activates protease and ring nuclease activities of CRISPR Lon-SAVED.
Journal, issue, pagesMol Cell, Vol. 84, Issue 21, Page 4239-44255.e8, Year 2024
Publish dateNov 7, 2024
AuthorsDalia Smalakyte / Audrone Ruksenaite / Giedrius Sasnauskas / Giedre Tamulaitiene / Gintautas Tamulaitis
PubMed AbstractTo combat phage infection, type III CRISPR-Cas systems utilize cyclic oligoadenylates (cA) signaling to activate various auxiliary effectors, including the CRISPR-associated Lon-SAVED protease CalpL, ...To combat phage infection, type III CRISPR-Cas systems utilize cyclic oligoadenylates (cA) signaling to activate various auxiliary effectors, including the CRISPR-associated Lon-SAVED protease CalpL, which forms a tripartite effector system together with an anti-σ factor, CalpT, and an ECF-like σ factor, CalpS. Here, we report the characterization of the Candidatus Cloacimonas acidaminovorans CalpL-CalpT-CalpS. We demonstrate that cA binding triggers CalpL filament formation and activates it to cleave CalpT within the CalpT-CalpS dimer. This cleavage exposes the CalpT C-degron, which targets it for further degradation by cellular proteases. Consequently, CalpS is released to bind to RNA polymerase, causing growth arrest in E. coli. Furthermore, the CalpL-CalpT-CalpS system is regulated by the SAVED domain of CalpL, which is a ring nuclease that cleaves cA in a sequential three-step mechanism. These findings provide key mechanistic details for the activation, proteolytic events, and regulation of the signaling cascade in the type III CRISPR-Cas immunity.
External linksMol Cell / PubMed:39362215
MethodsEM (single particle)
Resolution2.75 - 2.97 Å
Structure data

50054

9eyi

EMDB-50054, PDB-9eyi:
Cryo-EM structure of SAVED-Lon protease CCaCalpL filament bound to A4p
Method: EM (single particle) / Resolution: 2.75 Å

50055

9eyj

EMDB-50055, PDB-9eyj:
Cryo-EM structure of SAVED-Lon protease CCaCalpL filament bound to cA4
Method: EM (single particle) / Resolution: 2.97 Å

50056

9eyk

EMDB-50056, PDB-9eyk:
Cryo-EM structure of SAVED-Lon protease CCaCalpL filament bound to A4>p
Method: EM (single particle) / Resolution: 2.84 Å

Source
  • candidatus cloacimonas acidaminovorans (bacteria)
  • synthetic construct (others)
KeywordsHYDROLASE / LON PROTEASE / SAVED DOMAIN / CRISPR

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