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TitleStructural basis of archaeal FttA-dependent transcription termination.
Journal, issue, pagesNature, Year 2024
Publish dateSep 25, 2024
AuthorsLinlin You / Chengyuan Wang / Vadim Molodtsov / Konstantin Kuznedelov / Xinyi Miao / Breanna R Wenck / Paul Ulisse / Travis J Sanders / Craig J Marshall / Emre Firlar / Jason T Kaelber / Thomas J Santangelo / Richard H Ebright /
PubMed AbstractThe ribonuclease FttA (also known as aCPSF and aCPSF1) mediates factor-dependent transcription termination in archaea. Here we report the structure of a Thermococcus kodakarensis transcription pre- ...The ribonuclease FttA (also known as aCPSF and aCPSF1) mediates factor-dependent transcription termination in archaea. Here we report the structure of a Thermococcus kodakarensis transcription pre-termination complex comprising FttA, Spt4, Spt5 and a transcription elongation complex (TEC). The structure shows that FttA interacts with the TEC in a manner that enables RNA to proceed directly from the TEC RNA-exit channel to the FttA catalytic centre and that enables endonucleolytic cleavage of RNA by FttA, followed by 5'→3' exonucleolytic cleavage of RNA by FttA and concomitant 5'→3' translocation of FttA on RNA, to apply mechanical force to the TEC and trigger termination. The structure further reveals that Spt5 bridges FttA and the TEC, explaining how Spt5 stimulates FttA-dependent termination. The results reveal functional analogy between bacterial and archaeal factor-dependent termination, functional homology between archaeal and eukaryotic factor-dependent termination, and fundamental mechanistic similarities in factor-dependent termination in bacteria, archaea, and eukaryotes.
External linksNature / PubMed:39322680
MethodsEM (single particle)
Resolution2.2 - 2.67 Å
Structure data

44438

9bct

EMDB-44438, PDB-9bct:
Cryo-EM structure of Thermococcus kodakarensis FttA-dependent transcription pre-termination complex containing 44 nt RNA
Method: EM (single particle) / Resolution: 2.5 Å

44439

9bcu

EMDB-44439, PDB-9bcu:
Cryo-EM structure of Thermococcus kodakarensis FttA-dependent transcription pre-termination complex containing 52 nt RNA
Method: EM (single particle) / Resolution: 2.2 Å

EMDB-44454: Cryo-EM structure of Thermococcus kodakarensis FttA-dependent transcription pre-termination complex containing 44 nt RNA
Method: EM (single particle) / Resolution: 2.5 Å

EMDB-44455: Cryo-EM structure of Thermococcus kodakarensis FttA-dependent transcription pre-termination complex containing 52 nt RNA
Method: EM (single particle) / Resolution: 2.2 Å

EMDB-44649: Cryo-EM structure of Thermococcus kodakarensis FttA-dependent transcription pre-termination complex containing 44 nt RNA (local refinement map)
Method: EM (single particle) / Resolution: 2.67 Å

EMDB-44650: Cryo-EM structure of Thermococcus kodakarensis FttA-dependent transcription pre-termination complex containing 52 nt RNA (local refinement map)
Method: EM (single particle) / Resolution: 2.34 Å

Chemicals

ChemComp-MG:
Unknown entry

ChemComp-ZN:
Unknown entry

Source
  • thermococcus kodakarensis (archaea)
  • synthetic construct (others)
KeywordsTRANSCRIPTION / RNA polymerase / pre-termination complex / FttA / archaea

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