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-Structure paper
タイトル | Cell-to-cell interactions revealed by cryo-tomography of a DPANN co-culture system. |
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ジャーナル・号・ページ | Nat Commun, Vol. 15, Issue 1, Page 7066, Year 2024 |
掲載日 | 2024年8月16日 |
![]() | Matthew D Johnson / Doulin C Shepherd / Hiroyuki D Sakai / Manasi Mudaliyar / Arun Prasad Pandurangan / Francesca L Short / Paul D Veith / Nichollas E Scott / Norio Kurosawa / Debnath Ghosal / ![]() ![]() ![]() |
PubMed 要旨 | DPANN is a widespread and diverse group of archaea characterized by their small size, reduced genome, limited metabolic pathways, and symbiotic existence. Known DPANN species are predominantly ...DPANN is a widespread and diverse group of archaea characterized by their small size, reduced genome, limited metabolic pathways, and symbiotic existence. Known DPANN species are predominantly obligate ectosymbionts that depend on their host for proliferation. The structural and molecular details of host recognition, host-DPANN intercellular communication, and host adaptation in response to DPANN attachment remain unknown. Here, we use electron cryotomography (cryo-ET) to show that the Microcaldus variisymbioticus ARM-1 may interact with its host, Metallosphaera javensis AS-7 through intercellular proteinaceous nanotubes. Combining cryo-ET and sub-tomogram averaging, we show the in situ architectures of host and DPANN S-layers and the structures of the nanotubes in their primed and extended states. In addition, comparative proteomics and genomic analyses identified host proteomic changes in response to DPANN attachment. These results provide insights into the structural basis of host-DPANN communication and deepen our understanding of the host ectosymbiotic relationships. |
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手法 | EM (サブトモグラム平均) |
解像度 | 18.6 - 27.3 Å |
構造データ | ![]() EMDB-42578: Subtomogram average of the Metallosphaera javensis AS-7 Surface layer ![]() EMDB-42579: Subtomogram average of the the Metallosphaera javensis AS-7 primed nanotube |
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