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TitleCell-to-cell interactions revealed by cryo-tomography of a DPANN co-culture system.
Journal, issue, pagesNat Commun, Vol. 15, Issue 1, Page 7066, Year 2024
Publish dateAug 16, 2024
AuthorsMatthew D Johnson / Doulin C Shepherd / Hiroyuki D Sakai / Manasi Mudaliyar / Arun Prasad Pandurangan / Francesca L Short / Paul D Veith / Nichollas E Scott / Norio Kurosawa / Debnath Ghosal /
PubMed AbstractDPANN is a widespread and diverse group of archaea characterized by their small size, reduced genome, limited metabolic pathways, and symbiotic existence. Known DPANN species are predominantly ...DPANN is a widespread and diverse group of archaea characterized by their small size, reduced genome, limited metabolic pathways, and symbiotic existence. Known DPANN species are predominantly obligate ectosymbionts that depend on their host for proliferation. The structural and molecular details of host recognition, host-DPANN intercellular communication, and host adaptation in response to DPANN attachment remain unknown. Here, we use electron cryotomography (cryo-ET) to show that the Microcaldus variisymbioticus ARM-1 may interact with its host, Metallosphaera javensis AS-7 through intercellular proteinaceous nanotubes. Combining cryo-ET and sub-tomogram averaging, we show the in situ architectures of host and DPANN S-layers and the structures of the nanotubes in their primed and extended states. In addition, comparative proteomics and genomic analyses identified host proteomic changes in response to DPANN attachment. These results provide insights into the structural basis of host-DPANN communication and deepen our understanding of the host ectosymbiotic relationships.
External linksNat Commun / PubMed:39152123 / PubMed Central
MethodsEM (subtomogram averaging)
Resolution18.6 - 27.3 Å
Structure data

EMDB-42578: Subtomogram average of the Metallosphaera javensis AS-7 Surface layer
Method: EM (subtomogram averaging) / Resolution: 18.6 Å

EMDB-42579: Subtomogram average of the the Metallosphaera javensis AS-7 primed nanotube
Method: EM (subtomogram averaging) / Resolution: 27.3 Å

Source
  • Metallosphaera javensis (ex Sakai et al. 2022) (archaea)

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