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Structure paper

TitleUnveiling the molecular mechanisms of the type IX secretion system's response regulator: Structural and functional insights.
Journal, issue, pagesPnas Nexus, Vol. 3, Page pgae316-pgae316, Year 2024
Publish dateJul 6, 2023 (structure data deposition date)
AuthorsSaran, A. / Kim, H.M. / Manning, I. / Hancock, M.A. / Schmitz, C. / Madej, M. / Potempa, J. / Sola, M. / Trempe, J.F. / Zhu, Y. ...Saran, A. / Kim, H.M. / Manning, I. / Hancock, M.A. / Schmitz, C. / Madej, M. / Potempa, J. / Sola, M. / Trempe, J.F. / Zhu, Y. / Davey, M.E. / Zeytuni, N.
External linksPnas Nexus / PubMed:39139265
MethodsX-ray diffraction
Resolution2.1 - 2.85 Å
Structure data

PDB-8ted:
PorX primitive orthorhombic crystal form
Method: X-RAY DIFFRACTION / Resolution: 2.1 Å

PDB-8tef:
PorX primitive monoclinic crystal form
Method: X-RAY DIFFRACTION / Resolution: 2.85 Å

PDB-8tff:
PorX with BeF3 phosphate analog
Method: X-RAY DIFFRACTION / Resolution: 2.34 Å

PDB-8tfm:
PorX with Zn (primitive orthorhombic crystal form)
Method: X-RAY DIFFRACTION / Resolution: 2.72 Å

PDB-8thp:
PorX phosphatase null mutant (T271V)
Method: X-RAY DIFFRACTION / Resolution: 2.6 Å

Chemicals

ChemComp-BR:
BROMIDE ION

ChemComp-CA:
Unknown entry

ChemComp-ACT:
ACETATE ION

ChemComp-HOH:
WATER

ChemComp-SO4:
SULFATE ION

ChemComp-MG:
Unknown entry

ChemComp-BEF:
BERYLLIUM TRIFLUORIDE ION

ChemComp-ZN:
Unknown entry

ChemComp-GOL:
GLYCEROL

Source
  • flavobacterium johnsoniae uw101 (bacteria)
  • flavobacterium johnsoniae (strain atcc 17061 / dsm 2064 / jcm 8514 / nbrc 14942 / ncimb 11054 / uw101) (bacteria)
KeywordsSIGNALING PROTEIN / Type-IX secretion system / response regulator / alkaline phosphatase

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