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TitleCryo-EM structure of 1-deoxy-D-xylulose 5-phosphate synthase DXPS from Plasmodium falciparum reveals a distinct N-terminal domain.
Journal, issue, pagesNat Commun, Vol. 15, Issue 1, Page 6642, Year 2024
Publish dateAug 5, 2024
AuthorsVictor O Gawriljuk / Andre S Godoy / Rick Oerlemans / Luise A T Welker / Anna K H Hirsch / Matthew R Groves /
PubMed AbstractPlasmodium falciparum is the main causative agent of malaria, a deadly disease that mainly affects children under five years old. Artemisinin-based combination therapies have been pivotal in ...Plasmodium falciparum is the main causative agent of malaria, a deadly disease that mainly affects children under five years old. Artemisinin-based combination therapies have been pivotal in controlling the disease, but resistance has arisen in various regions, increasing the risk of treatment failure. The non-mevalonate pathway is essential for the isoprenoid synthesis in Plasmodium and provides several under-explored targets to be used in the discovery of new antimalarials. 1-deoxy-D-xylulose-5-phosphate synthase (DXPS) is the first and rate-limiting enzyme of the pathway. Despite its importance, there are no structures available for any Plasmodium spp., due to the complex sequence which contains large regions of high disorder, making crystallisation a difficult task. In this manuscript, we use cryo-electron microscopy to solve the P. falciparum DXPS structure at a final resolution of 2.42 Å. Overall, the structure resembles other DXPS enzymes but includes a distinct N-terminal domain exclusive to the Plasmodium genus. Mutational studies show that destabilization of the cap domain interface negatively impacts protein stability and activity. Additionally, a density for the co-factor thiamine diphosphate is found in the active site. Our work highlights the potential of cryo-EM to obtain structures of P. falciparum proteins that are unfeasible by means of crystallography.
External linksNat Commun / PubMed:39103329 / PubMed Central
MethodsEM (single particle)
Resolution2.42 Å
Structure data

18842

8r2h

EMDB-18842, PDB-8r2h:
Cryo-EM structure of 1-deoxy-D-xylulose 5-phosphate synthase (DXPS) from Plasmodium falciparum
Method: EM (single particle) / Resolution: 2.42 Å

Chemicals

ChemComp-MG:
Unknown entry

ChemComp-TPP:
THIAMINE DIPHOSPHATE

Source
  • plasmodium falciparum (malaria parasite P. falciparum)
KeywordsTRANSFERASE / 1-deoxy-D-xylulose 5-phosphate synthase / thiamin di-phosphate complex / transketolase

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