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-Structure paper
タイトル | Capsid structure of bacteriophage ΦKZ provides insights into assembly and stabilization of jumbo phages. |
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ジャーナル・号・ページ | Nat Commun, Vol. 15, Issue 1, Page 6551, Year 2024 |
掲載日 | 2024年8月2日 |
![]() | Yashan Yang / Qianqian Shao / Mingcheng Guo / Lin Han / Xinyue Zhao / Aohan Wang / Xiangyun Li / Bo Wang / Ji-An Pan / Zhenguo Chen / Andrei Fokine / Lei Sun / Qianglin Fang / ![]() ![]() |
PubMed 要旨 | Jumbo phages are a group of tailed bacteriophages with large genomes and capsids. As a prototype of jumbo phage, ΦKZ infects Pseudomonas aeruginosa, a multi-drug-resistant (MDR) opportunistic ...Jumbo phages are a group of tailed bacteriophages with large genomes and capsids. As a prototype of jumbo phage, ΦKZ infects Pseudomonas aeruginosa, a multi-drug-resistant (MDR) opportunistic pathogen leading to acute or chronic infection in immunocompromised individuals. It holds potential to be used as an antimicrobial agent and as a model for uncovering basic phage biology. Although previous low-resolution structural studies have indicated that jumbo phages may have more complicated capsid structures than smaller phages such as HK97, the detailed structures and the assembly mechanism of their capsids remain largely unknown. Here, we report a 3.5-Å-resolution cryo-EM structure of the ΦKZ capsid. The structure unveiled ten minor capsid proteins, with some decorating the outer surface of the capsid and the others forming a complex network attached to the capsid's inner surface. This network seems to play roles in driving capsid assembly and capsid stabilization. Similar mechanisms of capsid assembly and stabilization are probably employed by many other jumbo viruses. |
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手法 | EM (単粒子) |
解像度 | 3.5 - 5.4 Å |
構造データ | ![]()
EMDB-38845: Icosahedrally averaged cryo-EM reconstruction of PhiKZ capsid before applying the "block-based" reconstruction method ![]()
EMDB-38846: Block 1 of PhiKZ capsid ![]()
EMDB-38848: Block 2 of PhiKZ capsid EMDB-39002: Composite cryo-EM map of PhiKZ capsid after applying the "block-based" reconstruction method |
由来 |
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![]() | VIRUS / phiKZ / capsid protein / viral assembly / Pseudomonas aeruginosa / jumbo phage |