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TitleCapsid structure of bacteriophage ΦKZ provides insights into assembly and stabilization of jumbo phages.
Journal, issue, pagesNat Commun, Vol. 15, Issue 1, Page 6551, Year 2024
Publish dateAug 2, 2024
AuthorsYashan Yang / Qianqian Shao / Mingcheng Guo / Lin Han / Xinyue Zhao / Aohan Wang / Xiangyun Li / Bo Wang / Ji-An Pan / Zhenguo Chen / Andrei Fokine / Lei Sun / Qianglin Fang /
PubMed AbstractJumbo phages are a group of tailed bacteriophages with large genomes and capsids. As a prototype of jumbo phage, ΦKZ infects Pseudomonas aeruginosa, a multi-drug-resistant (MDR) opportunistic ...Jumbo phages are a group of tailed bacteriophages with large genomes and capsids. As a prototype of jumbo phage, ΦKZ infects Pseudomonas aeruginosa, a multi-drug-resistant (MDR) opportunistic pathogen leading to acute or chronic infection in immunocompromised individuals. It holds potential to be used as an antimicrobial agent and as a model for uncovering basic phage biology. Although previous low-resolution structural studies have indicated that jumbo phages may have more complicated capsid structures than smaller phages such as HK97, the detailed structures and the assembly mechanism of their capsids remain largely unknown. Here, we report a 3.5-Å-resolution cryo-EM structure of the ΦKZ capsid. The structure unveiled ten minor capsid proteins, with some decorating the outer surface of the capsid and the others forming a complex network attached to the capsid's inner surface. This network seems to play roles in driving capsid assembly and capsid stabilization. Similar mechanisms of capsid assembly and stabilization are probably employed by many other jumbo viruses.
External linksNat Commun / PubMed:39095371 / PubMed Central
MethodsEM (single particle)
Resolution3.5 - 5.4 Å
Structure data


EMDB entry, No image

EMDB-38845: Icosahedrally averaged cryo-EM reconstruction of PhiKZ capsid before applying the "block-based" reconstruction method
Method: EM (single particle) / Resolution: 5.4 Å


EMDB entry, No image

EMDB-38846: Block 1 of PhiKZ capsid
Method: EM (single particle) / Resolution: 3.5 Å


EMDB entry, No image

EMDB-38848: Block 2 of PhiKZ capsid
Method: EM (single particle) / Resolution: 3.6 Å

39002
EMDB entry, No image

8y6v

EMDB-39002: Composite cryo-EM map of PhiKZ capsid after applying the "block-based" reconstruction method
PDB-8y6v: Near-atomic structure of icosahedrally averaged jumbo bacteriophage PhiKZ capsid
Method: EM (single particle) / Resolution: 3.5 Å

Source
  • phikzvirus phikz
KeywordsVIRUS / phiKZ / capsid protein / viral assembly / Pseudomonas aeruginosa / jumbo phage

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