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TitleStructure and mechanism of lysosome transmembrane acetylation by HGSNAT.
Journal, issue, pagesNat Struct Mol Biol, Vol. 31, Issue 10, Page 1502-1508, Year 2024
Publish dateMay 20, 2024
AuthorsRuisheng Xu / Yingjie Ning / Fandong Ren / Chenxia Gu / Zhengjiang Zhu / Xuefang Pan / Alexey V Pshezhetsky / Jingpeng Ge / Jie Yu /
PubMed AbstractLysosomal transmembrane acetylation of heparan sulfates (HS) is catalyzed by HS acetyl-CoA:α-glucosaminide N-acetyltransferase (HGSNAT), whose dysfunction leads to lysosomal storage diseases. The ...Lysosomal transmembrane acetylation of heparan sulfates (HS) is catalyzed by HS acetyl-CoA:α-glucosaminide N-acetyltransferase (HGSNAT), whose dysfunction leads to lysosomal storage diseases. The mechanism by which HGSNAT, the sole non-hydrolase enzyme in HS degradation, brings cytosolic acetyl-coenzyme A (Ac-CoA) and lysosomal HS together for N-acyltransferase reactions remains unclear. Here, we present cryogenic-electron microscopy structures of HGSNAT alone, complexed with Ac-CoA and with acetylated products. These structures explain that Ac-CoA binding from the cytosolic side causes dimeric HGSNAT to form a transmembrane tunnel. Within this tunnel, catalytic histidine and asparagine approach the lumen and instigate the transfer of the acetyl group from Ac-CoA to the glucosamine group of HS. Our study unveils a transmembrane acetylation mechanism that may help advance therapeutic strategies targeting lysosomal storage diseases.
External linksNat Struct Mol Biol / PubMed:38769387
MethodsEM (single particle)
Resolution2.59 - 2.87 Å
Structure data

36376

8jkv

EMDB-36376, PDB-8jkv:
membrane proteins
Method: EM (single particle) / Resolution: 2.87 Å

36386

8jl1

EMDB-36386, PDB-8jl1:
membrane proteins
Method: EM (single particle) / Resolution: 2.8 Å

36387

8jl3

EMDB-36387, PDB-8jl3:
membrane proteins
Method: EM (single particle) / Resolution: 2.59 Å

37264

8w4a

EMDB-37264, PDB-8w4a:
membrane proteins
Method: EM (single particle) / Resolution: 2.69 Å

Chemicals

ChemComp-NAG:
2-acetamido-2-deoxy-beta-D-glucopyranose

ChemComp-D12:
DODECANE

ChemComp-OCT:
N-OCTANE

ChemComp-C14:
TETRADECANE

ChemComp-CLR:
CHOLESTEROL

ChemComp-D10:
DECANE

ChemComp-R16:
HEXADECANE

ChemComp-ACO:
ACETYL COENZYME *A


ChemComp, No image

ChemComp-UV6:
Unknown entry

ChemComp-HP6:
HEPTANE

Source
  • homo sapiens (human)
KeywordsMEMBRANE PROTEIN / enzyme / acetylation

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