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-Structure paper
タイトル | Structural characterization of Thogoto Virus nucleoprotein provides insights into viral RNA encapsidation and RNP assembly. |
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ジャーナル・号・ページ | Structure, Year 2024 |
掲載日 | 2024年5月3日 |
著者 | Alexej Dick / Vasilii Mikirtumov / Jonas Fuchs / Ferdinand Krupp / Daniel Olal / Elias Bendl / Thiemo Sprink / Christoph Diebolder / Mikhail Kudryashev / Georg Kochs / Yvette Roske / Oliver Daumke / |
PubMed 要旨 | Orthomyxoviruses, such as influenza and thogotoviruses, are important human and animal pathogens. Their segmented viral RNA genomes are wrapped by viral nucleoproteins (NPs) into helical ...Orthomyxoviruses, such as influenza and thogotoviruses, are important human and animal pathogens. Their segmented viral RNA genomes are wrapped by viral nucleoproteins (NPs) into helical ribonucleoprotein complexes (RNPs). NP structures of several influenza viruses have been reported. However, there are still contradictory models of how orthomyxovirus RNPs are assembled. Here, we characterize the crystal structure of Thogoto virus (THOV) NP and found striking similarities to structures of influenza viral NPs, including a two-lobed domain architecture, a positively charged RNA-binding cleft, and a tail loop important for trimerization and viral transcription. A low-resolution cryo-electron tomography reconstruction of THOV RNPs elucidates a left-handed double helical assembly. By providing a model for RNP assembly of THOV, our study suggests conserved NP assembly and RNA encapsidation modes for thogoto- and influenza viruses. |
リンク | Structure / PubMed:38749445 |
手法 | EM (サブトモグラム平均) / X線回折 |
解像度 | 2.01 - 18.0 Å |
構造データ | EMDB-19599, PDB-8ryt: PDB-8cjw: |
化合物 | ChemComp-SIN: ChemComp-EDO: ChemComp-PO4: ChemComp-HOH: |
由来 |
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キーワード | VIRAL PROTEIN / viral replication / nucleoprotein / RNA binding / orthomyxovirus / oligomerization |