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TitleStructural characterization of Thogoto Virus nucleoprotein provides insights into viral RNA encapsidation and RNP assembly.
Journal, issue, pagesStructure, Vol. 32, Issue 8, Page 1068-11078.e5, Year 2024
Publish dateAug 8, 2024
AuthorsAlexej Dick / Vasilii Mikirtumov / Jonas Fuchs / Ferdinand Krupp / Daniel Olal / Elias Bendl / Thiemo Sprink / Christoph Diebolder / Mikhail Kudryashev / Georg Kochs / Yvette Roske / Oliver Daumke /
PubMed AbstractOrthomyxoviruses, such as influenza and thogotoviruses, are important human and animal pathogens. Their segmented viral RNA genomes are wrapped by viral nucleoproteins (NPs) into helical ...Orthomyxoviruses, such as influenza and thogotoviruses, are important human and animal pathogens. Their segmented viral RNA genomes are wrapped by viral nucleoproteins (NPs) into helical ribonucleoprotein complexes (RNPs). NP structures of several influenza viruses have been reported. However, there are still contradictory models of how orthomyxovirus RNPs are assembled. Here, we characterize the crystal structure of Thogoto virus (THOV) NP and found striking similarities to structures of influenza viral NPs, including a two-lobed domain architecture, a positively charged RNA-binding cleft, and a tail loop important for trimerization and viral transcription. A low-resolution cryo-electron tomography reconstruction of THOV RNPs elucidates a left-handed double helical assembly. By providing a model for RNP assembly of THOV, our study suggests conserved NP assembly and RNA encapsidation modes for thogoto- and influenza viruses.
External linksStructure / PubMed:38749445
MethodsEM (subtomogram averaging) / X-ray diffraction
Resolution2.01 - 18.0 Å
Structure data

19599

8ryt

EMDB-19599, PDB-8ryt:
Structural characterization of Thogoto Virus nucleoprotein provides insights into RNA encapsidation and assembly
Method: EM (subtomogram averaging) / Resolution: 18.0 Å

PDB-8cjw:
Nucleoprotein Thogotovirus delta188-196
Method: X-RAY DIFFRACTION / Resolution: 2.01 Å

Chemicals

ChemComp-SIN:
SUCCINIC ACID

ChemComp-EDO:
1,2-ETHANEDIOL

ChemComp-PO4:
PHOSPHATE ION

ChemComp-HOH:
WATER

Source
  • thogotovirus
  • thogotovirus thogotoense
KeywordsVIRAL PROTEIN / viral replication / nucleoprotein / RNA binding / orthomyxovirus / oligomerization

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