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TitleSelected humanization of yeast U1 snRNP leads to global suppression of pre-mRNA splicing and mitochondrial dysfunction in the budding yeast.
Journal, issue, pagesRNA, Vol. 30, Issue 8, Page 1070-1088, Year 2024
Publish dateJul 16, 2024
AuthorsSubbaiah Chalivendra / Shasha Shi / Xueni Li / Zhiling Kuang / Joseph Giovinazzo / Lingdi Zhang / John Rossi / Jingxin Wang / Anthony J Saviola / Robb Welty / Shiheng Liu / Katherine F Vaeth / Z Hong Zhou / Kirk C Hansen / J Matthew Taliaferro / Rui Zhao /
PubMed AbstractThe recognition of the 5' splice site (5' ss) is one of the earliest steps of pre-mRNA splicing. To better understand, the mechanism and regulation of 5' ss recognition, we selectively humanized ...The recognition of the 5' splice site (5' ss) is one of the earliest steps of pre-mRNA splicing. To better understand, the mechanism and regulation of 5' ss recognition, we selectively humanized components of the yeast U1 (yU1) snRNP to reveal the function of these components in 5' ss recognition and splicing. We targeted U1C and Luc7, two proteins that interact with and stabilize the yU1 snRNA and the 5' ss RNA duplex. We replaced the zinc-finger (ZnF) domain of yeast U1C (yU1C) with its human counterpart, which resulted in a cold-sensitive growth phenotype and moderate splicing defects. We next added an auxin-inducible degron to yeast Luc7 (yLuc7) protein (to mimic the lack of Luc7Ls in human U1 snRNP). We found that Luc7-depleted yU1 snRNP resulted in the concomitant loss of Prp40 and Snu71 (two other essential yU1 snRNP proteins), and further biochemical analyses suggest a model of how these three proteins interact with each other in the U1 snRNP. The loss of these proteins resulted in a significant growth retardation accompanied by a global suppression of pre-mRNA splicing. The splicing suppression led to mitochondrial dysfunction as revealed by a release of Fe into the growth medium and an induction of mitochondrial reactive oxygen species. Together, these observations indicate that the human U1C ZnF can substitute that of yeast, Luc7 is essential for the incorporation of the Luc7-Prp40-Snu71 trimer into yU1 snRNP, and splicing plays a major role in the regulation of mitochondrial function in yeast.
External linksRNA / PubMed:38688558 / PubMed Central
MethodsEM (single particle)
Resolution3.49 Å
Structure data

43753

8w2o

EMDB-43753, PDB-8w2o:
Yeast U1 snRNP with humanized U1C Zinc-Finger domain
Method: EM (single particle) / Resolution: 3.49 Å

Source
  • saccharomyces cerevisiae s288c (yeast)
KeywordsSPLICING / U1snRNP / U1C humanization

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