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TitleCharacterization of the extended substrate spectrum of the class A beta-lactamase CESS-1 from Stenotrophomonas sp. and structure-based investigation into its substrate preference.
Journal, issue, pagesInt J Antimicrob Agents, Vol. 63, Page 107171-107171, Year 2024
Publish dateMar 21, 2023 (structure data deposition date)
AuthorsJeong, B.G. / Kim, M.Y. / Jeong, C.S. / Do, H. / Hwang, J. / Lee, J.H. / Cha, S.S.
External linksInt J Antimicrob Agents / PubMed:38588869
MethodsX-ray diffraction
Resolution1.29 - 2.24 Å
Structure data

PDB-8iso:
Crystal structure of extended-spectrum class A beta-lactamase, CESS-1
Method: X-RAY DIFFRACTION / Resolution: 1.29 Å

PDB-8isp:
Crystal structure of extended-spectrum class A beta-lactamase, CESS-1 E166Q acylated by cephalexin
Method: X-RAY DIFFRACTION / Resolution: 2.11 Å

PDB-8isq:
Crystal structure of extended-spectrum class A beta-lactamase, CESS-1 E166Q acylated by ampicillin
Method: X-RAY DIFFRACTION / Resolution: 2.24 Å

PDB-8isr:
Crystal structure of extended-spectrum class A beta-lactamase, CESS-1 E166Q acylated by cefaclor
Method: X-RAY DIFFRACTION / Resolution: 2.09 Å

Chemicals

ChemComp-EDO:
1,2-ETHANEDIOL

ChemComp-PG5:
1-METHOXY-2-[2-(2-METHOXY-ETHOXY]-ETHANE

ChemComp-HOH:
WATER


ChemComp, No image

ChemComp-Q53:
Unknown entry

ChemComp-ZZ7:
(2R,4S)-2-[(R)-{[(2R)-2-amino-2-phenylacetyl]amino}(carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid

ChemComp-BTB:
2-[BIS-(2-HYDROXY-ETHYL)-AMINO]-2-HYDROXYMETHYL-PROPANE-1,3-DIOL / pH buffer*YM

ChemComp-GOL:
GLYCEROL


ChemComp, No image

ChemComp-Q6R:
Unknown entry

Source
  • stenotrophomonas sp. kctc 12332 (bacteria)
KeywordsHYDROLASE / extended-spectrum Class A beta-lactamase

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