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TitleStructure of the human outer kinetochore KMN network complex.
Journal, issue, pagesNat Struct Mol Biol, Vol. 31, Issue 6, Page 874-883, Year 2024
Publish dateMar 8, 2024
AuthorsStanislau Yatskevich / Jing Yang / Dom Bellini / Ziguo Zhang / David Barford /
PubMed AbstractFaithful chromosome segregation requires robust, load-bearing attachments of chromosomes to the mitotic spindle, a function accomplished by large macromolecular complexes termed kinetochores. In most ...Faithful chromosome segregation requires robust, load-bearing attachments of chromosomes to the mitotic spindle, a function accomplished by large macromolecular complexes termed kinetochores. In most eukaryotes, the constitutive centromere-associated network (CCAN) complex of the inner kinetochore recruits to centromeres the ten-subunit outer kinetochore KMN network that comprises the KNL1C, MIS12C and NDC80C complexes. The KMN network directly attaches CCAN to microtubules through MIS12C and NDC80C. Here, we determined a high-resolution cryo-EM structure of the human KMN network. This showed an intricate and extensive assembly of KMN subunits, with the central MIS12C forming rigid interfaces with NDC80C and KNL1C, augmented by multiple peptidic inter-subunit connections. We also observed that unphosphorylated MIS12C exists in an auto-inhibited state that suppresses its capacity to interact with CCAN. Ser100 and Ser109 of the N-terminal segment of the MIS12C subunit Dsn1, two key targets of Aurora B kinase, directly stabilize this auto-inhibition. Our study indicates how selectively relieving this auto-inhibition through Ser100 and Ser109 phosphorylation might restrict outer kinetochore assembly to functional centromeres during cell division.
External linksNat Struct Mol Biol / PubMed:38459127 / PubMed Central
MethodsEM (single particle)
Resolution3.0 Å
Structure data

17814

8ppr

EMDB-17814, PDB-8ppr:
Structure of the human outer kinetochore KMN network complex
Method: EM (single particle) / Resolution: 3.0 Å

Chemicals

ChemComp-HOH:
WATER

Source
  • homo sapiens (human)
KeywordsCELL CYCLE / Kinetochore / complex / KMN

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