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Structure paper

TitleCryo-EM structure of the inactive ribosome complex accumulated in chick embryo cells in cold-stress conditions.
Journal, issue, pagesFEBS Lett, Vol. 598, Issue 5, Page 537-547, Year 2024
Publish dateFeb 23, 2024
AuthorsLiliia Nurullina / Salvatore Terrosu / Alexander G Myasnikov / Lasse Bohl Jenner / Marat Yusupov /
PubMed AbstractHere, we present the high-resolution structure of the Gallus gallus 80S ribosome obtained from cold-treated chicken embryos. The translationally inactive ribosome complex contains elongation factor ...Here, we present the high-resolution structure of the Gallus gallus 80S ribosome obtained from cold-treated chicken embryos. The translationally inactive ribosome complex contains elongation factor eEF2 with GDP, SERPINE1 mRNA binding protein 1 (SERBP1) and deacylated tRNA in the P/E position, showing common features with complexes already described in mammals. Modeling of most expansion segments of G. gallus 28S ribosomal RNA allowed us to identify specific features in their structural organization and to describe areas where a marked difference between mammalian and avian ribosomes could shed light on the evolution of the expansion segments. This study provides the first structure of an avian ribosome, establishing a model for future structural and functional studies on the translational machinery in Aves.
External linksFEBS Lett / PubMed:38395592
MethodsEM (single particle)
Resolution2.4 - 3.0 Å
Structure data

EMDB-18158: Sharpened map of the Gallus gallus 80S rotated ribosome in complex with eEF2 and SERBP1 (cryoSPARC)
Method: EM (single particle) / Resolution: 2.4 Å

EMDB-18159: Focused map of the 40S ribosomal subunit head of the Gallus gallus (rotated)
Method: EM (single particle) / Resolution: 2.9 Å

EMDB-18160: Focused map of the 40S ribosomal subunit body of the Gallus gallus (rotated)
Method: EM (single particle) / Resolution: 2.7 Å

EMDB-18161: Focused map of the 60S ribosomal subunit ot the Gallus gallus
Method: EM (single particle) / Resolution: 2.4 Å

EMDB-18165: Sharpened map of the Gallus gallus 80S non-rotated ribosome (cryoSPARC)
Method: EM (single particle) / Resolution: 2.5 Å

EMDB-18166: Focused map of the 40S ribosomal subunit head of the Gallus gallus (non-rotated)
Method: EM (single particle) / Resolution: 3.0 Å

EMDB-18167: Focused map of the 40S ribosomal subunit body of the Gallus gallus (non-rotated)
Method: EM (single particle) / Resolution: 2.9 Å

18168

8q7z

EMDB-18168: Composite map of the Gallus gallus 80S non-rotated ribosome
PDB-8q7z: Structure of the G. gallus 80S non-rotated ribosome
Method: EM (single particle) / Resolution: 2.5 Å

18169

8q87

EMDB-18169: Composite map of the Gallus gallus 80S rotated ribosome in complex with eEF2 and SERBP1
PDB-8q87: Structure of the G. gallus 80S rotated ribosome in complex with eEF2 and SERBP1
Method: EM (single particle) / Resolution: 2.4 Å

Chemicals

ChemComp-MG:
Unknown entry

ChemComp-ZN:
Unknown entry

ChemComp-HOH:
WATER

ChemComp-GDP:
GUANOSINE-5'-DIPHOSPHATE / GDP, energy-carrying molecule*YM

Source
  • gallus gallus (chicken)
KeywordsRIBOSOME / translation / complex / eEF2 / SERBP1

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