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TitleElucidation of unusual biosynthesis and DnaN-targeting mode of action of potent anti-tuberculosis antibiotics Mycoplanecins.
Journal, issue, pagesNat Commun, Vol. 15, Page 791-791, Year 2024
Publish dateFeb 11, 2023 (structure data deposition date)
AuthorsFu, C. / Liu, Y. / Walt, C. / Rasheed, S. / Bader, C.D. / Lukat, P. / Neuber, M. / Haeckl, F.P.J. / Blankenfeldt, W. / Kalinina, O.V. / Muller, R.
External linksNat Commun / PubMed:38278788
MethodsX-ray diffraction
Resolution1.54 - 2.27 Å
Structure data

PDB-8cix:
DNA-polymerase sliding clamp (DnaN) from Escherichia coli in complex with Griselimycin.
Method: X-RAY DIFFRACTION / Resolution: 1.76 Å

PDB-8ciy:
DNA-polymerase sliding clamp (DnaN) from Escherichia coli in complex with Cyclohexyl-Griselimycin.
Method: X-RAY DIFFRACTION / Resolution: 1.54 Å

PDB-8ciz:
DNA-polymerase sliding clamp (DnaN) from Escherichia coli in complex with Mycoplanecin A.
Method: X-RAY DIFFRACTION / Resolution: 2.27 Å

Chemicals

ChemComp-GOL:
GLYCEROL

ChemComp-CA:
Unknown entry

ChemComp-ACT:
ACETATE ION

ChemComp-MG:
Unknown entry

ChemComp-NA:
Unknown entry

ChemComp-HOH:
WATER

Source
  • escherichia coli (E. coli)
  • streptomyces griseus (bacteria)
  • synthetic construct (others)
  • actinoplanes awajinensis subsp. mycoplanecinus (bacteria)
KeywordsTRANSFERASE / DnaN / Sliding clamp / DNA-polymerase beta subunit / Antibiotic / Natural product / Anti-tuberculosis

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