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| Title | Graphene sandwich-based biological specimen preparation for cryo-EM analysis. |
|---|---|
| Journal, issue, pages | Proc Natl Acad Sci U S A, Vol. 121, Issue 5, Page e2309384121, Year 2024 |
| Publish date | Jan 30, 2024 |
 Authors | Jie Xu / Xiaoyin Gao / Liming Zheng / Xia Jia / Kui Xu / Yuwei Ma / Xiaoding Wei / Nan Liu / Hailin Peng / Hong-Wei Wang /  |
| PubMed Abstract | High-quality specimen preparation plays a crucial role in cryo-electron microscopy (cryo-EM) structural analysis. In this study, we have developed a reliable and convenient technique called the ...High-quality specimen preparation plays a crucial role in cryo-electron microscopy (cryo-EM) structural analysis. In this study, we have developed a reliable and convenient technique called the graphene sandwich method for preparing cryo-EM specimens. This method involves using two layers of graphene films that enclose macromolecules on both sides, allowing for an appropriate ice thickness for cryo-EM analysis. The graphene sandwich helps to mitigate beam-induced charging effect and reduce particle motion compared to specimens prepared using the traditional method with graphene support on only one side, therefore improving the cryo-EM data quality. These advancements may open new opportunities to expand the use of graphene in the field of biological electron microscopy. |
 External links | Proc Natl Acad Sci U S A / PubMed:38252835 / PubMed Central |
| Methods | EM (single particle) |
| Resolution | 2.5 - 3.1 Å |
| Structure data |  EMDB-37156: Graphene sandwiched equine spleen apoferritin  EMDB-37158: Graphene sandwiched 20S proteasome  EMDB-38213: Graphene sandwiched spike |
| Source |
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Equus caballus (horse)
Thermoplasma acidophilum (acidophilic)
Severe acute respiratory syndrome coronavirus 2