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-Structure paper
タイトル | A unified Watson-Crick geometry drives transcription of six-letter expanded DNA alphabets by E. coli RNA polymerase. |
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ジャーナル・号・ページ | Nat Commun, Vol. 14, Issue 1, Page 8219, Year 2023 |
掲載日 | 2023年12月12日 |
著者 | Juntaek Oh / Zelin Shan / Shuichi Hoshika / Jun Xu / Jenny Chong / Steven A Benner / Dmitry Lyumkis / Dong Wang / |
PubMed 要旨 | Artificially Expanded Genetic Information Systems (AEGIS) add independently replicable unnatural nucleotide pairs to the natural G:C and A:T/U pairs found in native DNA, joining the unnatural pairs ...Artificially Expanded Genetic Information Systems (AEGIS) add independently replicable unnatural nucleotide pairs to the natural G:C and A:T/U pairs found in native DNA, joining the unnatural pairs through alternative modes of hydrogen bonding. Whether and how AEGIS pairs are recognized and processed by multi-subunit cellular RNA polymerases (RNAPs) remains unknown. Here, we show that E. coli RNAP selectively recognizes unnatural nucleobases in a six-letter expanded genetic system. High-resolution cryo-EM structures of three RNAP elongation complexes containing template-substrate UBPs reveal the shared principles behind the recognition of AEGIS and natural base pairs. In these structures, RNAPs are captured in an active state, poised to perform the chemistry step. At this point, the unnatural base pair adopts a Watson-Crick geometry, and the trigger loop is folded into an active conformation, indicating that the mechanistic principles underlying recognition and incorporation of natural base pairs also apply to AEGIS unnatural base pairs. These data validate the design philosophy of AEGIS unnatural basepairs. Further, we provide structural evidence supporting a long-standing hypothesis that pair mismatch during transcription occurs via tautomerization. Together, our work highlights the importance of Watson-Crick complementarity underlying the design principles of AEGIS base pair recognition. |
リンク | Nat Commun / PubMed:38086811 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 2.65 - 3.28 Å |
構造データ | EMDB-40862, PDB-8sy5: EMDB-40863, PDB-8sy6: EMDB-40864, PDB-8sy7: |
化合物 | ChemComp-ZN: ChemComp-MG: ChemComp-X0F: ChemComp-HOH: ChemComp-DGP: ChemComp-UTP: ChemComp-X0O: |
由来 |
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キーワード | TRANSCRIPTION/DNA/RNA / complex / unnatural base pairs / synthetic biology / transcription / TRANSCRIPTION-DNA-RNA complex |