EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structural basis of peptide secretion for Quorum sensing by ComA.
ジャーナル・号・ページ	Nat Commun, Vol. 14, Issue 1, Page 7178, Year 2023
掲載日	2023年11月7日
著者	Lin Yu / Xin Xu / Wan-Zhen Chua / Hao Feng / Zheng Ser / Kai Shao / Jian Shi / Yumei Wang / Zongli Li / Radoslaw M Sobota / Lok-To Sham / Min Luo /
PubMed 要旨	Quorum sensing (QS) is a crucial regulatory mechanism controlling bacterial signalling and holds promise for novel therapies against antimicrobial resistance. In Gram-positive bacteria, such as ...Quorum sensing (QS) is a crucial regulatory mechanism controlling bacterial signalling and holds promise for novel therapies against antimicrobial resistance. In Gram-positive bacteria, such as Streptococcus pneumoniae, ComA is a conserved efflux pump responsible for the maturation and secretion of peptide signals, including the competence-stimulating peptide (CSP), yet its structure and function remain unclear. Here, we functionally characterize ComA as an ABC transporter with high ATP affinity and determined its cryo-EM structures in the presence or absence of CSP or nucleotides. Our findings reveal a network of strong electrostatic interactions unique to ComA at the intracellular gate, a putative binding pocket for two CSP molecules, and negatively charged residues facilitating CSP translocation. Mutations of these residues affect ComA's peptidase activity in-vitro and prevent CSP export in-vivo. We demonstrate that ATP-Mg triggers the outward-facing conformation of ComA for CSP release, rather than ATP alone. Our study provides molecular insights into the QS signal peptide secretion, highlighting potential targets for QS-targeting drugs.
リンク	Nat Commun / PubMed:37935699 / PubMed Central
手法	EM (単粒子)
解像度	2.8 - 8.2 Å
構造データ	34712 8hf4 EMDB-34712, PDB-8hf4: Cryo-EM structure of nucleotide-bound ComA at outward-facing state with EC gate closed conformation 手法: EM (単粒子) / 解像度: 2.8 Å 34713 8hf5 EMDB-34713, PDB-8hf5: Cryo-EM structure of nucleotide-bound ComA at outward-facing state with EC gate open conformation 手法: EM (単粒子) / 解像度: 2.9 Å 34714 8hf6 EMDB-34714, PDB-8hf6: Cryo-EM structure of nucleotide-bound ComA E647Q mutant 手法: EM (単粒子) / 解像度: 3.1 Å 34715 8hf7 EMDB-34715, PDB-8hf7: Cryo-EM structure of ComA bound to its mature substrate CSP peptide 手法: EM (単粒子) / 解像度: 3.8 Å EMDB-34716: Cryo-EM structure of ComC bound ComA C17A at inward-facing state 手法: EM (単粒子) / 解像度: 8.2 Å 36882 8k4b EMDB-36882, PDB-8k4b: Cryo-EM structure of nucleotide-bound ComA with ZinC ion 手法: EM (単粒子) / 解像度: 3.9 Å 36936 8k7a EMDB-36936, PDB-8k7a: Cryo-EM structure of nucleotide-bound ComA E647Q mutant with Mg2+ 手法: EM (単粒子) / 解像度: 4.2 Å
化合物	ChemComp-AGS: PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER / ATP-γ-S / ATP-gamma-S, エネルギー貯蔵分子類似体YM ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-ATP: ADENOSINE-5'-TRIPHOSPHATE / ATP / ATP, エネルギー貯蔵分子YM ChemComp-ZN: Unknown entry
由来	streptococcus pneumoniae d39 (肺炎レンサ球菌) streptococcus streptococcus pneumoniae d39 (肺炎レンサ球菌) Streptococcus pneumoniae (肺炎レンサ球菌) streptococcus pneumoniae r6 (肺炎レンサ球菌)
キーワード	MEMBRANE PROTEIN / TRANSPORT PROTEIN / ABC transport / PCAT / HYDROLASE / TRANSLOCASE / ABC transporter