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Structure paper

TitleIrreversible inactivation of lactate racemase by sodium borohydride reveals reactivity of the nickel-pincer nucleotide cofactor.
Journal, issue, pagesAcs Catalysis, Vol. 13, Page 1441-1448, Year 2023
Publish dateOct 31, 2022 (structure data deposition date)
AuthorsGatreddi, S. / Sui, D. / Hausinger, R.P. / Hu, J.
External linksAcs Catalysis / PubMed:37886035
MethodsX-ray diffraction
Resolution1.99 - 2.15 Å
Structure data

PDB-8ezf:
A tethered niacin-derived pincer complex with a nickel-carbon or sulfite-carbon bond in lactate racemase R98A/R100A variant
Method: X-RAY DIFFRACTION / Resolution: 2.15 Å

PDB-8ezh:
A tethered niacin-derived pincer complex with a nickel-carbon bond in lactate racemase R98A/R100A variant modeled with sulfite-NPN adduct
Method: X-RAY DIFFRACTION / Resolution: 1.99 Å

PDB-8ezi:
A tethered niacin-derived pincer complex with a nickel-carbon bond in lactate racemase R98A/R100A variant modeled with separated sulfite and NPN
Method: X-RAY DIFFRACTION / Resolution: 1.99 Å

Chemicals

ChemComp-CA:
Unknown entry

ChemComp-MG:
Unknown entry

ChemComp-CL:
Unknown entry

ChemComp-ENJ:
(4S)-5-methanethioyl-1-(5-O-phosphono-beta-D-ribofuranosyl)-4-sulfo-1,4-dihydropyridine-3-carbothioic S-acid

ChemComp-NI:
NICKEL (II) ION

ChemComp-EDO:
1,2-ETHANEDIOL

ChemComp-HOH:
WATER

ChemComp-SO3:
SULFITE ION

ChemComp-4EY:
1-[(2R,3R,4S,5R)-3,4-bis(oxidanyl)-5-(phosphonooxymethyl)oxolan-2-yl]-5-methanethioyl-pyridine-3-carbothioic S-acid

Source
  • lactiplantibacillus plantarum wcfs1 (bacteria)
KeywordsISOMERASE / Catalytic activity / isomerase activity / racemase and epimerase activity racemase acting on hydroxy acids and derivatives / metal ion binding

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