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Structure paper

TitleRep protein accommodates together dsDNA and ssDNA which enables a loop-back mechanism to plasmid DNA replication initiation.
Journal, issue, pagesNucleic Acids Res., Vol. 51, Page 10551-10567, Year 2023
Publish dateJul 1, 2022 (structure data deposition date)
AuthorsWegrzyn, K. / Oliwa, M. / Nowacka, M. / Zabrocka, E. / Bury, K. / Purzycki, P. / Czaplewska, P. / Pipka, J. / Giraldo, R. / Konieczny, I.
External linksNucleic Acids Res. / PubMed:37713613
MethodsX-ray diffraction
Resolution1.6 - 2.19 Å
Structure data

PDB-8aan:
The nucleoprotein complex of Rep protein with iteron containing dsDNA and DUE ssDNA.
Method: X-RAY DIFFRACTION / Resolution: 2.19 Å

PDB-8ac8:
The nucleoprotein complex of Rep protein with DUE ssDNA
Method: X-RAY DIFFRACTION / Resolution: 1.6 Å

Chemicals

ChemComp-MG:
Unknown entry

ChemComp-HOH:
WATER

Source
  • escherichia coli (E. coli)
  • synthetic construct (others)
KeywordsDNA BINDING PROTEIN / Replication initiation protein Nucleoprotein complex Nucleic acid binding DNA binding

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