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Structure paper

TitleManganese Transfer Hydrogenases Based on the Biotin-Streptavidin Technology.
Journal, issue, pagesAngew. Chem. Int. Ed. Engl., Vol. 62, Page e202311896-e202311896, Year 2023
Publish dateMay 24, 2023 (structure data deposition date)
AuthorsWang, W. / Tachibana, R. / Zou, Z. / Chen, D. / Zhang, X. / Lau, K. / Pojer, F. / Ward, T.R. / Hu, X.
External linksAngew. Chem. Int. Ed. Engl. / PubMed:37671593
MethodsX-ray diffraction
Resolution1.56 - 1.88 Å
Structure data

PDB-8p5y:
Artificial transfer hydrogenase with a Mn-12 cofactor and Streptavidin S112Y-K121M mutant
Method: X-RAY DIFFRACTION / Resolution: 1.88 Å

PDB-8p5z:
Artificial transfer hydrogenase with a Mn-5 cofactor and Streptavidin S112Y-K121M mutant
Method: X-RAY DIFFRACTION / Resolution: 1.56 Å

Chemicals

ChemComp-WZQ:
5-[(3~{a}~{S},4~{S},6~{a}~{R})-2-oxidanylidene-1,3,3~{a},4,6,6~{a}-hexahydrothieno[3,4-d]imidazol-4-yl]-~{N}-[2-(3,4-dihydro-2~{H}-pyrano[2,3-c]pyridin-6-ylmethylamino)ethyl]pentanamide

ChemComp-MN:
Unknown entry

ChemComp-BR:
BROMIDE ION

ChemComp-CL:
Unknown entry

ChemComp-HOH:
WATER

ChemComp-X08:
5-[(3~{a}~{S},4~{S},6~{a}~{R})-2-oxidanylidene-1,3,3~{a},4,6,6~{a}-hexahydrothieno[3,4-d]imidazol-4-yl]-~{N}-[2-[(5-methylpyridin-2-yl)methylamino]ethyl]pentanamide

ChemComp-GOL:
GLYCEROL

Source
  • streptomyces avidinii (bacteria)
KeywordsMETAL BINDING PROTEIN / Artificial meatlloenzyme / Transfer hydrogenase / Manganese

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