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Structure paper

TitleRedox driven B 12 -ligand switch drives CarH photoresponse.
Journal, issue, pagesNat Commun, Vol. 14, Page 5082-5082, Year 2023
Publish dateDec 23, 2022 (structure data deposition date)
AuthorsPoddar, H. / Rios-Santacruz, R. / Heyes, D.J. / Shanmugam, M. / Brookfield, A. / Johannissen, L.O. / Levy, C.W. / Jeffreys, L.N. / Zhang, S. / Sakuma, M. ...Poddar, H. / Rios-Santacruz, R. / Heyes, D.J. / Shanmugam, M. / Brookfield, A. / Johannissen, L.O. / Levy, C.W. / Jeffreys, L.N. / Zhang, S. / Sakuma, M. / Colletier, J.P. / Hay, S. / Schiro, G. / Weik, M. / Scrutton, N.S. / Leys, D.
External linksNat Commun / PubMed:37604813
MethodsX-ray diffraction
Resolution1.7 - 2.5 Å
Structure data

PDB-8c31:
Dark state 1.8 Angstrom crystal structure of cobalamin binding domain belonging to a light-dependent transcription regulator TtCarH obtained under aerobic condition
Method: X-RAY DIFFRACTION / Resolution: 1.8 Å

PDB-8c32:
Dark state 2.2 Angstrom crystal structure of cobalamin binding domain belonging to a light-dependent transcription regulator TtCarH obtained under anaerobic condition
Method: X-RAY DIFFRACTION / Resolution: 2.2 Å

PDB-8c33:
Anaerobic light exposed 2.25 Angstrom crystal structure of cobalamin binding domain belonging to a light-dependent transcription regulator TtCarH
Method: X-RAY DIFFRACTION / Resolution: 2.25 Å

PDB-8c34:
Aerobic light exposed 1.8 Angstrom crystal structure of cobalamin binding domain belonging to a light-dependent transcription regulator TtCarH
Method: X-RAY DIFFRACTION / Resolution: 1.82 Å

PDB-8c35:
Dark state 2.1 Angstrom crystal structure of H132A variant of cobalamin binding domain belonging to a light-dependent transcription regulator TtCarH
Method: X-RAY DIFFRACTION / Resolution: 2.1 Å

PDB-8c36:
Light-adapted 2.0 Angstrom crystal structure of H132A variant of cobalamin binding domain belonging to a light-dependent transcription regulator TtCarH obtained under aerobic conditions
Method: X-RAY DIFFRACTION / Resolution: 2 Å

PDB-8c37:
An intermediate light exposed 2.15 Angstrom crystal structure of H132A variant of cobalamin binding domain belonging to a light-dependent transcription regulator TtCarH obtained under anaerobic conditions
Method: X-RAY DIFFRACTION / Resolution: 2.15 Å

PDB-8c73:
Dark state 1.8 Angstrom crystal structure of cobalamin binding domain belonging to a light-dependent transcription regulator TtCarH obtained under aerobic condition form ll
Method: X-RAY DIFFRACTION / Resolution: 1.7 Å

PDB-8c76:
Light-state 2.5 Angstrom wild-type X-ray crystal structure of the cobalamin binding domain belonging to a light-dependent transcription regulator TtCarH obtained under aerobic conditions from a form 2 crystal illuminated during 5 s
Method: X-RAY DIFFRACTION / Resolution: 2.5 Å

Chemicals

ChemComp-BR:
BROMIDE ION

ChemComp-B12:
COBALAMIN

ChemComp-5AD:
5'-DEOXYADENOSINE

ChemComp-IMD:
IMIDAZOLE

ChemComp-PGE:
TRIETHYLENE GLYCOL

ChemComp-EDO:
1,2-ETHANEDIOL

ChemComp-HOH:
WATER

ChemComp-PEG:
DI(HYDROXYETHYL)ETHER

Source
  • thermus thermophilus hb27 (bacteria)
  • thermus thermophilus (bacteria)
KeywordsTRANSCRIPTION / CarH / cobalamin / light-activated / transcription regulator / anaerobic protein crystallography / anaerobic / DNA BINDING PROTEIN / B12 / Adocobalamin / Adenosylcobalamine / photoreceptor / light-sensitive protein

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