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TitleStructure determination of a DNA crystal by MicroED.
Journal, issue, pagesStructure, Vol. 31, Issue 12, Page 1499-11503.e2, Year 2023
Publish dateDec 7, 2023
AuthorsAlison Haymaker / Andrey A Bardin / Tamir Gonen / Michael W Martynowycz / Brent L Nannenga /
PubMed AbstractMicrocrystal electron diffraction (MicroED) is a powerful tool for determining high-resolution structures of microcrystals from a diverse array of biomolecular, chemical, and material samples. In ...Microcrystal electron diffraction (MicroED) is a powerful tool for determining high-resolution structures of microcrystals from a diverse array of biomolecular, chemical, and material samples. In this study, we apply MicroED to DNA crystals, which have not been previously analyzed using this technique. We utilized the d(CGCGCG) DNA duplex as a model sample and employed cryo-FIB milling to create thin lamella for diffraction data collection. The MicroED data collection and subsequent processing resulted in a 1.10 Å resolution structure of the d(CGCGCG) DNA, demonstrating the successful application of cryo-FIB milling and MicroED to the investigation of nucleic acid crystals.
External linksStructure / PubMed:37541248 / PubMed Central
MethodsEM (electron crystallography)
Resolution1.1 Å
Structure data

PDB-8skw:
MicroED structure of d(CGCGCG)2 Z-DNA
Method: ELECTRON CRYSTALLOGRAPHY / Resolution: 1.1 Å

Chemicals

ChemComp-SPM:
SPERMINE

ChemComp-HOH:
WATER

Source
  • synthetic construct (others)
KeywordsDNA / Z-DNA

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