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TitleWeiTsing, a pericycle-expressed ion channel, safeguards the stele to confer clubroot resistance.
Journal, issue, pagesCell, Vol. 186, Issue 12, Page 2656-2671.e18, Year 2023
Publish dateJun 8, 2023
AuthorsWei Wang / Li Qin / Wenjing Zhang / Linghui Tang / Chao Zhang / Xiaojing Dong / Pei Miao / Meng Shen / Huilong Du / Hangyuan Cheng / Ke Wang / Xiangyun Zhang / Min Su / Hongwei Lu / Chang Li / Qiang Gao / Xiaojuan Zhang / Yun Huang / Chengzhi Liang / Jian-Min Zhou / Yu-Hang Chen /
PubMed AbstractPlant roots encounter numerous pathogenic microbes that often cause devastating diseases. One such pathogen, Plasmodiophora brassicae (Pb), causes clubroot disease and severe yield losses on ...Plant roots encounter numerous pathogenic microbes that often cause devastating diseases. One such pathogen, Plasmodiophora brassicae (Pb), causes clubroot disease and severe yield losses on cruciferous crops worldwide. Here, we report the isolation and characterization of WeiTsing (WTS), a broad-spectrum clubroot resistance gene from Arabidopsis. WTS is transcriptionally activated in the pericycle upon Pb infection to prevent pathogen colonization in the stele. Brassica napus carrying the WTS transgene displayed strong resistance to Pb. WTS encodes a small protein localized in the endoplasmic reticulum (ER), and its expression in plants induces immune responses. The cryoelectron microscopy (cryo-EM) structure of WTS revealed a previously unknown pentameric architecture with a central pore. Electrophysiology analyses demonstrated that WTS is a calcium-permeable cation-selective channel. Structure-guided mutagenesis indicated that channel activity is strictly required for triggering defenses. The findings uncover an ion channel analogous to resistosomes that triggers immune signaling in the pericycle.
External linksCell / PubMed:37295403
MethodsEM (single particle)
Resolution4.14 Å
Structure data

34710

8hf2

EMDB-34710, PDB-8hf2:
Cryo-EM structure of WeiTsing
Method: EM (single particle) / Resolution: 4.14 Å

Source
  • arabidopsis thaliana (thale cress)
KeywordsMEMBRANE PROTEIN / Pentamer / Channel

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