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TitleModular droplet injector for sample conservation providing new structural insight for the conformational heterogeneity in the disease-associated NQO1 enzyme.
Journal, issue, pagesLab Chip, Vol. 23, Page 3016-3033, Year 2023
Publish dateJan 23, 2023 (structure data deposition date)
AuthorsDoppler, D. / Sonker, M. / Egatz-Gomez, A. / Grieco, A. / Zaare, S. / Jernigan, R. / Meza-Aguilar, J.D. / Rabbani, M.T. / Manna, A. / Alvarez, R.C. ...Doppler, D. / Sonker, M. / Egatz-Gomez, A. / Grieco, A. / Zaare, S. / Jernigan, R. / Meza-Aguilar, J.D. / Rabbani, M.T. / Manna, A. / Alvarez, R.C. / Karpos, K. / Cruz Villarreal, J. / Nelson, G. / Yang, J.H. / Carrion, J. / Morin, K. / Ketawala, G.K. / Pey, A.L. / Ruiz-Fresneda, M.A. / Pacheco-Garcia, J.L. / Hermoso, J.A. / Nazari, R. / Sierra, R. / Hunter, M.S. / Batyuk, A. / Kupitz, C.J. / Sublett, R.E. / Lisova, S. / Mariani, V. / Boutet, S. / Fromme, R. / Grant, T.D. / Botha, S. / Fromme, P. / Kirian, R.A. / Martin-Garcia, J.M. / Ros, A.
External linksLab Chip / PubMed:37294576
MethodsX-ray diffraction
Resolution2 - 2.7 Å
Structure data

PDB-8c9j:
Crystal structure of human NQO1 by serial femtosecond crystallography
Method: X-RAY DIFFRACTION / Resolution: 2.7 Å

PDB-8fwa:
Phycocyanin structure from a modular droplet injector for serial femtosecond crystallography
Method: X-RAY DIFFRACTION / Resolution: 2 Å

Chemicals

ChemComp-FAD:
FLAVIN-ADENINE DINUCLEOTIDE / FAD*YM

ChemComp-ACT:
ACETATE ION

ChemComp-HOH:
WATER

ChemComp-CYC:
PHYCOCYANOBILIN

ChemComp-NA:
Unknown entry

Source
  • homo sapiens (human)
  • thermosynechococcus vestitus bp-1 (bacteria)
KeywordsOXIDOREDUCTASE / NQO1 / flavoprotein / cancer / serial femtosecond crystallography / microcrystals / XFEL / Droplet injection / PLANT PROTEIN / Pigment Protein / segmented flow injector

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