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-Structure paper
タイトル | Fast and versatile sequence-independent protein docking for nanomaterials design using RPXDock. |
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ジャーナル・号・ページ | PLoS Comput Biol, Vol. 19, Issue 5, Page e1010680, Year 2023 |
掲載日 | 2023年5月22日 |
著者 | William Sheffler / Erin C Yang / Quinton Dowling / Yang Hsia / Chelsea N Fries / Jenna Stanislaw / Mark D Langowski / Marisa Brandys / Zhe Li / Rebecca Skotheim / Andrew J Borst / Alena Khmelinskaia / Neil P King / David Baker / |
PubMed 要旨 | Computationally designed multi-subunit assemblies have shown considerable promise for a variety of applications, including a new generation of potent vaccines. One of the major routes to such ...Computationally designed multi-subunit assemblies have shown considerable promise for a variety of applications, including a new generation of potent vaccines. One of the major routes to such materials is rigid body sequence-independent docking of cyclic oligomers into architectures with point group or lattice symmetries. Current methods for docking and designing such assemblies are tailored to specific classes of symmetry and are difficult to modify for novel applications. Here we describe RPXDock, a fast, flexible, and modular software package for sequence-independent rigid-body protein docking across a wide range of symmetric architectures that is easily customizable for further development. RPXDock uses an efficient hierarchical search and a residue-pair transform (RPX) scoring method to rapidly search through multidimensional docking space. We describe the structure of the software, provide practical guidelines for its use, and describe the available functionalities including a variety of score functions and filtering tools that can be used to guide and refine docking results towards desired configurations. |
リンク | PLoS Comput Biol / PubMed:37216343 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 3.67 Å |
構造データ | EMDB-29502, PDB-8fwd: |
由来 |
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キーワード | DE NOVO PROTEIN / octahedra / oligomer / de novo design / rosetta / cryoEM / interface |