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TitleStructural and biochemical analysis of a novel atypically split intein reveals a conserved histidine specific to cysteine-less inteins.
Journal, issue, pagesChem Sci, Vol. 14, Page 5204-5213, Year 2023
Publish dateMar 3, 2023 (structure data deposition date)
AuthorsPasch, T. / Schroder, A. / Kattelmann, S. / Eisenstein, M. / Pietrokovski, S. / Kummel, D. / Mootz, H.D.
External linksChem Sci / PubMed:37206380
MethodsX-ray diffraction
Resolution1.85 - 2.6 Å
Structure data

PDB-8cpn:
Crystal structure of the PolB16_OarG intein variant S1A, N183A
Method: X-RAY DIFFRACTION / Resolution: 1.85 Å

PDB-8cpo:
Crystal structure of the PolB16_OarG intein variant S1A, N183A, C111A, C165A
Method: X-RAY DIFFRACTION / Resolution: 2.6 Å

Chemicals

ChemComp-IOD:
IODIDE ION

ChemComp-HOH:
WATER

Source
  • metagenome (others)
KeywordsSPLICING / split intein / ligation / protein engineering

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