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-Structure paper
タイトル | Protein-protein interactions in the Mla lipid transport system probed by computational structure prediction and deep mutational scanning. |
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ジャーナル・号・ページ | J Biol Chem, Vol. 299, Issue 6, Page 104744, Year 2023 |
掲載日 | 2023年4月25日 |
![]() | Mark R MacRae / Dhenesh Puvanendran / Max A B Haase / Nicolas Coudray / Ljuvica Kolich / Cherry Lam / Minkyung Baek / Gira Bhabha / Damian C Ekiert / ![]() |
PubMed 要旨 | The outer membrane (OM) of Gram-negative bacteria is an asymmetric bilayer that protects the cell from external stressors, such as antibiotics. The Mla transport system is implicated in the ...The outer membrane (OM) of Gram-negative bacteria is an asymmetric bilayer that protects the cell from external stressors, such as antibiotics. The Mla transport system is implicated in the Maintenance of OM Lipid Asymmetry by mediating retrograde phospholipid transport across the cell envelope. Mla uses a shuttle-like mechanism to move lipids between the MlaFEDB inner membrane complex and the MlaA-OmpF/C OM complex, via a periplasmic lipid-binding protein, MlaC. MlaC binds to MlaD and MlaA, but the underlying protein-protein interactions that facilitate lipid transfer are not well understood. Here, we take an unbiased deep mutational scanning approach to map the fitness landscape of MlaC from Escherichia coli, which provides insights into important functional sites. Combining this analysis with AlphaFold2 structure predictions and binding experiments, we map the MlaC-MlaA and MlaC-MlaD protein-protein interfaces. Our results suggest that the MlaD and MlaA binding surfaces on MlaC overlap to a large extent, leading to a model in which MlaC can only bind one of these proteins at a time. Low-resolution cryo-electron microscopy (cryo-EM) maps of MlaC bound to MlaFEDB suggest that at least two MlaC molecules can bind to MlaD at once, in a conformation consistent with AlphaFold2 predictions. These data lead us to a model for MlaC interaction with its binding partners and insights into lipid transfer steps that underlie phospholipid transport between the bacterial inner and OMs. |
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手法 | EM (単粒子) |
解像度 | 6.6 - 7.8 Å |
構造データ | ![]() EMDB-29041: E. coli MlaFEDB bound to MlaC ![]() EMDB-40162: E. coli MlaC bound to MlaFEDB |
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