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TitleDefective import of mitochondrial metabolic enzyme elicits ectopic metabolic stress.
Journal, issue, pagesSci Adv, Vol. 9, Page eadf1956-eadf1956, Year 2023
Publish dateAug 31, 2022 (structure data deposition date)
AuthorsNishio, K. / Kawarasaki, T. / Sugiura, Y. / Matsumoto, S. / Konoshima, A. / Takano, Y. / Hayashi, M. / Okumura, F. / Kamura, T. / Mizushima, T. / Nakatsukasa, K.
External linksSci Adv / PubMed:37058555
MethodsX-ray diffraction
Resolution1.42 - 2.53 Å
Structure data

PDB-8gqz:
Crystal structure of mitochondrial citrate synthase (Cit1) from Saccharomyces cerevisiae
Method: X-RAY DIFFRACTION / Resolution: 1.42 Å

PDB-8gr8:
Crystal structure of peroxisomal citrate synthase (Cit2) from Saccharomycescerevisiae
Method: X-RAY DIFFRACTION / Resolution: 2.39 Å

PDB-8gr9:
Crystal structure of peroxisomal citrate synthase (Cit2) from Saccharomyces cerevisiae in complex with oxaloacetate and coenzyme-A
Method: X-RAY DIFFRACTION / Resolution: 1.48 Å

PDB-8gre:
F-box protein in complex with skp1(FL) and substrate
Method: X-RAY DIFFRACTION / Resolution: 2.3 Å

PDB-8grf:
Crystal structure of F-box protein in the ternary complex with adaptor protein Skp1(DL) and its substrate
Method: X-RAY DIFFRACTION / Resolution: 2.53 Å

Chemicals

ChemComp-NA:
Unknown entry

ChemComp-CL:
Unknown entry

ChemComp-ACT:
ACETATE ION

ChemComp-GOL:
GLYCEROL

ChemComp-HOH:
WATER

ChemComp-EPE:
4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID / pH buffer*YM

ChemComp-PEG:
DI(HYDROXYETHYL)ETHER

ChemComp-COA:
COENZYME A

ChemComp-K:
Unknown entry

ChemComp-OAA:
OXALOACETATE ION

ChemComp-EDO:
1,2-ETHANEDIOL

Source
  • saccharomyces cerevisiae (brewer's yeast)
KeywordsTRANSFERASE / TCA cycle / mitochondrial protein / citrate metabolism / SCFUcc1 ubiquitin ligase / proteasome-dependent degradation / glyoxylate cycle / peroxisomal protein / TRANSFERASE/LIGASE / F-box protein / E3 ubiquitin ligase / LIGASE / TRANSFERASE-LIGASE complex

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