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Structure paper

TitleEngineering mutually orthogonal PylRS/tRNA pairs for dual encoding of functional histidine analogues.
Journal, issue, pagesProtein Sci., Vol. 32, Page e4640-e4640, Year 2023
Publish dateJan 3, 2023 (structure data deposition date)
AuthorsTaylor, C.J. / Hardy, F.J. / Burke, A.J. / Bednar, R.M. / Mehl, R.A. / Green, A.P. / Lovelock, S.L.
External linksProtein Sci. / PubMed:37051694
MethodsX-ray diffraction
Resolution1.82 Å
Structure data

PDB-8c49:
Crystal structure of pyrrolysyl-tRNA synthetase from Methanomethylophilus alvus engineered for 3-Methyl-L-histidine, bound to AMPPNP
Method: X-RAY DIFFRACTION / Resolution: 1.82 Å

Chemicals

ChemComp-ANP:
PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER / AMP-PNP, energy-carrying molecule analogue*YM

ChemComp-PEG:
DI(HYDROXYETHYL)ETHER

ChemComp-PGE:
TRIETHYLENE GLYCOL

ChemComp-EDO:
1,2-ETHANEDIOL

ChemComp-MG:
Unknown entry

ChemComp-HOH:
WATER

Source
  • candidatus methanomethylophilus alvus (archaea)
KeywordsLIGASE / pyrrolysyl-tRNA synthetase / genetic code expansion / engineered translation components / Methanomethylophilus alvus

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