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TitleThe Crystal Structure of Engineered Nitroreductase NTR 2.0 and Impact of F70A and F108Y Substitutions on Substrate Specificity.
Journal, issue, pagesInt J Mol Sci, Vol. 24, Year 2023
Publish dateMay 3, 2022 (structure data deposition date)
AuthorsSharrock, A.V. / Mumm, J.S. / Bagdziunas, G. / Cenas, N. / Arcus, V.L. / Ackerley, D.F.
External linksInt J Mol Sci / PubMed:37047605
MethodsX-ray diffraction
Resolution1.85 Å
Structure data

PDB-7uwt:
Structure of Oxygen-Insensitive NAD(P)H-dependent Nitroreductase NfsB_Vv F70A/F108Y (NTR 2.0) in complex with FMN at 1.85 Angstroms resolution
Method: X-RAY DIFFRACTION / Resolution: 1.85 Å

Chemicals

ChemComp-FMN:
FLAVIN MONONUCLEOTIDE

ChemComp-ACT:
ACETATE ION

ChemComp-P4K:
polyethylene glycol

ChemComp-HOH:
WATER

Source
  • vibrio vulnificus (bacteria)
KeywordsOXIDOREDUCTASE / nitroreductase / flavoenzyme / metronidazole reductase

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