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TitleEngineering C-C Bond Cleavage Activity into a P450 Monooxygenase Enzyme.
Journal, issue, pagesJ. Am. Chem. Soc., Vol. 145, Page 9207-9222, Year 2023
Publish dateFeb 6, 2023 (structure data deposition date)
AuthorsMiller, J.C. / Lee, J.H.Z. / Mclean, M.A. / Chao, R.R. / Stone, I.S.J. / Pukala, T.L. / Bruning, J.B. / De Voss, J.J. / Schuler, M.A. / Sligar, S.G. / Bell, S.G.
External linksJ. Am. Chem. Soc. / PubMed:37042073
MethodsX-ray diffraction
Resolution2 - 2.1 Å
Structure data

PDB-8g35:
Crystal structure of F182L-CYP199A4 in complex with (S)-4-(2-hydroxy-3-oxobutan-2-yl)benzoic acid
Method: X-RAY DIFFRACTION / Resolution: 2 Å

PDB-8g36:
Crystal structure of F182L-CYP199A4 in complex with terephthalic acid
Method: X-RAY DIFFRACTION / Resolution: 2.1 Å

Chemicals

ChemComp-HEM:
PROTOPORPHYRIN IX CONTAINING FE

ChemComp-YJE:
4-[(2S)-2-hydroxy-3-oxobutan-2-yl]benzoic acid

ChemComp-CL:
Unknown entry

ChemComp-HOH:
WATER

ChemComp-UB7:
terephthalic acid

Source
  • rhodopseudomonas palustris haa2 (phototrophic)
KeywordsOXIDOREDUCTASE / cytochrome / bacterial P450

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