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TitleStructure of the type III-D CRISPR effector.
Journal, issue, pagesCurr Res Struct Biol, Vol. 5, Page 100098, Year 2023
Publish dateFeb 10, 2023
AuthorsGiuseppe Cannone / Dmytro Kompaniiets / Shirley Graham / Malcolm F White / Laura Spagnolo /
PubMed AbstractCRISPR-Cas is a prokaryotic adaptive immune system, classified into six different types, each characterised by a signature protein. Type III systems, classified based on the presence of a Cas10 ...CRISPR-Cas is a prokaryotic adaptive immune system, classified into six different types, each characterised by a signature protein. Type III systems, classified based on the presence of a Cas10 subunit, are rather diverse multi-subunit assemblies with a range of enzymatic activities and downstream ancillary effectors. The broad array of current biotechnological CRISPR applications is mainly based on proteins classified as Type II, however recent developments established the feasibility and efficacy of multi-protein Type III CRISPR-Cas effector complexes as RNA-targeting tools in eukaryotes. The crenarchaeon has two type III system subtypes (III-B and III-D). Here, we report the cryo-EM structure of the Csm Type III-D complex from (SsoCsm), which uses CRISPR RNA to bind target RNA molecules, activating the Cas10 subunit for antiviral defence. The structure reveals the complex organisation, subunit/subunit connectivity and protein/guide RNA interactions of the SsoCsm complex, one of the largest CRISPR effectors known.
External linksCurr Res Struct Biol / PubMed:36843655 / PubMed Central
MethodsEM (single particle)
Resolution3.5 - 4.0 Å
Structure data

16126

8bmw

EMDB-16126, PDB-8bmw:
SsoCsm
Method: EM (single particle) / Resolution: 3.5 Å

EMDB-16174: Saccharolobus solfataricus type III-D CRISPR effector - Body 1
Method: EM (single particle) / Resolution: 3.5 Å

EMDB-16175: Saccharolobus solfataricus type III-D CRISPR effector body 2
Method: EM (single particle) / Resolution: 3.5 Å

EMDB-16176: Saccharolobus solfataricus type III-D CRISPR effector body 3
Method: EM (single particle) / Resolution: 3.5 Å

EMDB-16177: Saccharolobus solfataricus type III-D CRISPR effector Body 4
Method: EM (single particle) / Resolution: 3.5 Å

EMDB-16679: SsoCsm consensus
Method: EM (single particle) / Resolution: 4.0 Å

Source
  • saccharolobus solfataricus (archaea)
KeywordsRNA BINDING PROTEIN / Sulfolobus / Type III CRISPR

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