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TitleYet Another Similarity between Mitochondrial and Bacterial Ribosomal Small Subunit Biogenesis Obtained by Structural Characterization of RbfA from .
Journal, issue, pagesInt J Mol Sci, Vol. 24, Issue 3, Year 2023
Publish dateJan 20, 2023
AuthorsAydar G Bikmullin / Bulat Fatkhullin / Artem Stetsenko / Azat Gabdulkhakov / Natalia Garaeva / Liliia Nurullina / Evelina Klochkova / Alexander Golubev / Iskander Khusainov / Natalie Trachtmann / Dmitriy Blokhin / Albert Guskov / Shamil Validov / Konstantin Usachev / Marat Yusupov /
PubMed AbstractRibosome biogenesis is a complex and highly accurate conservative process of ribosomal subunit maturation followed by association. Subunit maturation comprises sequential stages of ribosomal RNA and ...Ribosome biogenesis is a complex and highly accurate conservative process of ribosomal subunit maturation followed by association. Subunit maturation comprises sequential stages of ribosomal RNA and proteins' folding, modification and binding, with the involvement of numerous RNAses, helicases, GTPases, chaperones, RNA, protein-modifying enzymes, and assembly factors. One such assembly factor involved in bacterial 30S subunit maturation is ribosomal binding factor A (RbfA). In this study, we present the crystal (determined at 2.2 Å resolution) and NMR structures of RbfA as well as the 2.9 Å resolution cryo-EM reconstruction of the 30S-RbfA complex from (). Additionally, we show that the manner of RbfA action on the small ribosomal subunit during its maturation is shared between bacteria and mitochondria. The obtained results clarify the function of RbfA in the 30S maturation process and its role in ribosome functioning in general. Furthermore, given that is a serious human pathogen, this study provides an additional prospect to develop antimicrobials targeting bacterial pathogens.
External linksInt J Mol Sci / PubMed:36768442 / PubMed Central
MethodsEM (single particle) / X-ray diffraction
Resolution2.22 - 2.89 Å
Structure data

16334

8byv

EMDB-16334, PDB-8byv:
Cryo-EM structure of a Staphylococus aureus 30S-RbfA complex
Method: EM (single particle) / Resolution: 2.89 Å

PDB-8bxa:
Crystal structure of ribosome binding factor A (RbfA) from S. aureus
Method: X-RAY DIFFRACTION / Resolution: 2.22 Å

Chemicals

ChemComp-HOH:
WATER

Source
  • staphylococcus aureus subsp. aureus nctc 8325 (bacteria)
KeywordsRIBOSOME / maturation / 30S subunit / S. aureus / RbfA / 30S subunit maturation / cryo-EM / ribosome biogenesis / translation / Staphylococcus aureus

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