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TitleBivalent intra-spike binding provides durability against emergent Omicron lineages: Results from a global consortium.
Journal, issue, pagesCell Rep, Vol. 42, Issue 1, Page 112014, Year 2023
Publish dateJan 31, 2023
AuthorsHeather M Callaway / Kathryn M Hastie / Sharon L Schendel / Haoyang Li / Xiaoying Yu / Jeremy Shek / Tierra Buck / Sean Hui / Dan Bedinger / Camille Troup / S Moses Dennison / Kan Li / Michael D Alpert / Charles C Bailey / Sharon Benzeno / Jody L Bonnevier / Jin-Qiu Chen / Charm Chen / Hyeseon Cho / Peter D Crompton / Vincent Dussupt / Kevin C Entzminger / Yassine Ezzyat / Jonathan K Fleming / Nick Geukens / Amy E Gilbert / Yongjun Guan / Xiaojian Han / Christopher J Harvey / Julia M Hatler / Bryan Howie / Chao Hu / Ailong Huang / Maya Imbrechts / Aishun Jin / Nik Kamachi / Gladys Keitany / Mark Klinger / Jay K Kolls / Shelly J Krebs / Tingting Li / Feiyan Luo / Toshiaki Maruyama / Michael A Meehl / Letzibeth Mendez-Rivera / Andrea Musa / C J Okumura / Benjamin E R Rubin / Aaron K Sato / Meiying Shen / Anirudh Singh / Shuyi Song / Joshua Tan / Jeffrey M Trimarchi / Dhruvkumar P Upadhyay / Yingming Wang / Lei Yu / Tom Z Yuan / Erik Yusko / Bjoern Peters / Georgia Tomaras / Erica Ollmann Saphire /
PubMed AbstractThe SARS-CoV-2 Omicron variant of concern (VoC) and its sublineages contain 31-36 mutations in spike and escape neutralization by most therapeutic antibodies. In a pseudovirus neutralization assay, ...The SARS-CoV-2 Omicron variant of concern (VoC) and its sublineages contain 31-36 mutations in spike and escape neutralization by most therapeutic antibodies. In a pseudovirus neutralization assay, 66 of the nearly 400 candidate therapeutics in the Coronavirus Immunotherapeutic Consortium (CoVIC) panel neutralize Omicron and multiple Omicron sublineages. Among natural immunoglobulin Gs (IgGs), especially those in the receptor-binding domain (RBD)-2 epitope community, nearly all Omicron neutralizers recognize spike bivalently, with both antigen-binding fragments (Fabs) simultaneously engaging adjacent RBDs on the same spike. Most IgGs that do not neutralize Omicron bind either entirely monovalently or have some (22%-50%) monovalent occupancy. Cleavage of bivalent-binding IgGs to Fabs abolishes neutralization and binding affinity, with disproportionate loss of activity against Omicron pseudovirus and spike. These results suggest that VoC-resistant antibodies overcome mutagenic substitution via avidity. Hence, vaccine strategies targeting future SARS-CoV-2 variants should consider epitope display with spacing and organization identical to trimeric spike.
External linksCell Rep / PubMed:36681898 / PubMed Central
MethodsEM (single particle)
Resolution16.0 - 26.0 Å
Structure data

EMDB-28090: Negative stain EM map of SARS-CoV-2 Spike in complex with CoVIC-040
Method: EM (single particle) / Resolution: 16.3 Å

EMDB-28091: Negative stain EM map of SARS-CoV-2 Spike in complex with CoVIC-045
Method: EM (single particle) / Resolution: 18.0 Å

EMDB-28092: Negative stain EM map of SARS-CoV-2 Spike in complex with CoVIC-093
Method: EM (single particle) / Resolution: 16.0 Å

EMDB-28093: Negative stain EM map of SARS-CoV-2 Spike in complex with CoVIC-156
Method: EM (single particle) / Resolution: 18.0 Å

EMDB-28094: Negative stain EM map of SARS-CoV-2 Spike in complex with CoVIC-234
Method: EM (single particle) / Resolution: 25.0 Å

EMDB-28095: Negative stain EM map of SARS-CoV-2 Spike in complex with CoVIC-260
Method: EM (single particle) / Resolution: 20.0 Å

EMDB-28096: Negative stain EM map of SARS-CoV-2 Spike in complex with CoVIC-279
Method: EM (single particle) / Resolution: 26.0 Å

EMDB-28097: Negative stain EM map of SARS-CoV-2 Spike in complex with CoVIC-290
Method: EM (single particle) / Resolution: 22.0 Å

EMDB-28098: Negative stain EM map of SARS-CoV-2 Spike in complex with CoVIC-294
Method: EM (single particle) / Resolution: 17.0 Å

EMDB-28099: Negative stain EM map of SARS-CoV-2 Spike in complex with CoVIC-295
Method: EM (single particle) / Resolution: 20.0 Å

EMDB-28100: Negative stain EM map of SARS-CoV-2 Spike in complex with CoVIC-299
Method: EM (single particle) / Resolution: 18.0 Å

EMDB-28102: Negative stain EM map of SARS-CoV-2 Spike in complex with CoVIC-334
Method: EM (single particle) / Resolution: 25.0 Å

EMDB-28103: Negative stain EM map of SARS-CoV-2 Spike in complex with CoVIC-360
Method: EM (single particle) / Resolution: 21.0 Å

EMDB-28104: Negative stain EM map of SARS-CoV-2 Spike in complex with CoVIC-361
Method: EM (single particle) / Resolution: 16.0 Å

EMDB-28105: Negative stain EM map of SARS-CoV-2 Spike in complex with CoVIC-362
Method: EM (single particle) / Resolution: 17.0 Å

EMDB-28106: Negative stain EM map of SARS-CoV-2 Spike in complex with CoVIC-368
Method: EM (single particle) / Resolution: 16.0 Å

EMDB-28168: Negative stain EM map of SARS-CoV-2 Spike in complex with CoVIC-292
Method: EM (single particle) / Resolution: 17.0 Å

EMDB-28169: Negative stain EM map of SARS-CoV-2 Spike in complex with CoVIC-333
Method: EM (single particle) / Resolution: 20.0 Å

EMDB-28170: Negative stain EM map of SARS-CoV-2 Spike in complex with CoVIC-355
Method: EM (single particle) / Resolution: 16.0 Å

EMDB-28171: Negative stain EM map of SARS-CoV-2 Spike in complex with CoVIC-371
Method: EM (single particle) / Resolution: 18.0 Å

Source
  • Severe acute respiratory syndrome coronavirus 2

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