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Structure paper

TitleEnolpyruvate transferase MurAA A149E , identified during adaptation of Enterococcus faecium to daptomycin, increases stability of MurAA-MurG interaction.
Journal, issue, pagesJ. Biol. Chem., Vol. 299, Page 102912-102912, Year 2023
Publish dateDec 21, 2021 (structure data deposition date)
AuthorsZhou, Y. / Utama, B. / Pratap, S. / Supandy, A. / Song, X. / Tran, T.T. / Mehta, H.H. / Arias, C.A. / Shamoo, Y.
External linksJ. Biol. Chem. / PubMed:36649910
MethodsX-ray diffraction
Resolution1.65 - 2.65 Å
Structure data

PDB-7tb0:
E. faecium MurAA in complex with fosfomycin and UNAG
Method: X-RAY DIFFRACTION / Resolution: 1.65 Å

PDB-8d84:
E. faecium MurAA in complex with UDP-N-acetylmuramic acid (UNAM) and a covalent adduct of PEP with Cys119
Method: X-RAY DIFFRACTION / Resolution: 2.65 Å

Chemicals

ChemComp-UD1:
URIDINE-DIPHOSPHATE-N-ACETYLGLUCOSAMINE

ChemComp-FFQ:
[(1R)-1-hydroxypropyl]phosphonic acid / antibiotic*YM

ChemComp-K:
Unknown entry

ChemComp-NA:
Unknown entry

ChemComp-CL:
Unknown entry

ChemComp-HOH:
WATER

ChemComp-EPZ:
(2R)-2-{[(2R,3R,4R,5S,6R)-3-(acetylamino)-2-{[(S)-{[(R)-{[(2R,3S,4R,5R)-5-(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methoxy}(hydroxy)phosphoryl]oxy}(hydroxy)phosphoryl]oxy}-5-hydroxy-6-(hydroxymethyl)tetrahydro-2H-pyran-4-yl]oxy}propanoic acid

Source
  • enterococcus faecium (bacteria)
  • enterococcus faecalis (bacteria)
KeywordsTRANSFERASE / MurAA / Enterococcus faecium

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