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TitleBrominated lipid probes expose structural asymmetries in constricted membranes.
Journal, issue, pagesNat Struct Mol Biol, Vol. 30, Issue 2, Page 167-175, Year 2023
Publish dateJan 9, 2023
AuthorsFrank R Moss / James Lincoff / Maxwell Tucker / Arshad Mohammed / Michael Grabe / Adam Frost /
PubMed AbstractLipids in biological membranes are thought to be functionally organized, but few experimental tools can probe nanoscale membrane structure. Using brominated lipids as contrast probes for cryo-EM and ...Lipids in biological membranes are thought to be functionally organized, but few experimental tools can probe nanoscale membrane structure. Using brominated lipids as contrast probes for cryo-EM and a model ESCRT-III membrane-remodeling system composed of human CHMP1B and IST1, we observed leaflet-level and protein-localized structural lipid patterns within highly constricted and thinned membrane nanotubes. These nanotubes differed markedly from protein-free, flat bilayers in leaflet thickness, lipid diffusion rates and lipid compositional and conformational asymmetries. Simulations and cryo-EM imaging of brominated stearoyl-docosahexanenoyl-phosphocholine showed how a pair of phenylalanine residues scored the outer leaflet with a helical hydrophobic defect where polyunsaturated docosahexaenoyl tails accumulated at the bilayer surface. Combining cryo-EM of halogenated lipids with molecular dynamics thus enables new characterizations of the composition and structure of membranes on molecular length scales.
External linksNat Struct Mol Biol / PubMed:36624348 / PubMed Central
MethodsEM (helical sym.)
Resolution2.8 - 9.5 Å
Structure data

EMDB-27991: CryoEM reconstruction of membrane-bound, left-handed CHMP1B+IST1 filament with brominated SDPC probe
Method: EM (helical sym.) / Resolution: 2.9 Å

EMDB-28694: CryoEM reconstruction of membrane-bound, right-handed CHMP1B+IST1 with brominated SDPC probe
Method: EM (helical sym.) / Resolution: 3.0 Å

EMDB-28695: CryoEM reconstruction of membrane-bound, left-handed CHMP1B+IST1 filament with brominated POPS probe
Method: EM (helical sym.) / Resolution: 2.8 Å

EMDB-28696: CryoEM reconstruction of membrane-bound, right-handed CHMP1B+IST1 filament with brominated POPS probe
Method: EM (helical sym.) / Resolution: 3.1 Å

EMDB-28697: CryoEM reconstruction of membrane-bound, left-handed CHMP1B+IST1 filament with brominated PIP2 probe
Method: EM (helical sym.) / Resolution: 3.2 Å

EMDB-28698: CryoEM reconstruction of membrane-bound, right-handed CHMP1B+IST1 filament with brominated PIP2 probe
Method: EM (helical sym.) / Resolution: 3.5 Å

EMDB-28699: CryoEM reconstruction of membrane-bound, left-handed CHMP1B+IST1 filament with brominated cholesterol probe
Method: EM (helical sym.) / Resolution: 3.8 Å

EMDB-28700: CryoEM reconstruction of membrane-bound, left-handed CHMP1B(F9L/F13L)+IST1 filament with no brominated lipids
Method: EM (helical sym.) / Resolution: 3.1 Å

EMDB-28701: CryoEM reconstruction of membrane-bound, right-handed CHMP1B+IST1 filament with no brominated lipids
Method: EM (helical sym.) / Resolution: 3.7 Å

EMDB-28702: CryoEM reconstruction of membrane-bound, left-handed CHMP1B+IST1 filament with iodinated cholesterol probe
Method: EM (helical sym.) / Resolution: 3.2 Å

EMDB-28703: CryoEM reconstruction of membrane-bound, right-handed CHMP1B+IST1 filament with iodinated cholesterol probe
Method: EM (helical sym.) / Resolution: 3.1 Å

EMDB-28704: CryoEM reconstruction of membrane-bound, left-handed CHMP1B+IST1 filament with 25% brominated SDPC probe
Method: EM (helical sym.) / Resolution: 3.1 Å

EMDB-28705: CryoEM reconstruction of membrane-bound, right-handed CHMP1B+IST1 filament with 25% brominated SDPC probe
Method: EM (helical sym.) / Resolution: 2.9 Å

EMDB-28706: CryoEM reconstruction of membrane-bound, left-handed CHMP1B+IST1 filament with 50% brominated SDPC probe
Method: EM (helical sym.) / Resolution: 2.9 Å

EMDB-28707: CryoEM reconstruction of membrane-bound, right-handed CHMP1B+IST1 filament with 50% brominated SDPC probe
Method: EM (helical sym.) / Resolution: 3.0 Å

EMDB-28708: CryoEM reconstruction of membrane-bound, left-handed CHMP1B+IST1 filament with 0% cholesterol
Method: EM (helical sym.) / Resolution: 4.1 Å

EMDB-28709: CryoEM reconstruction of membrane-bound, left-handed CHMP1B+IST1 filament with 10% cholesterol
Method: EM (helical sym.) / Resolution: 4.0 Å

EMDB-28710: CryoEM reconstruction of membrane-bound, left-handed CHMP1B+IST1 filament with 20% cholesterol
Method: EM (helical sym.) / Resolution: 7.6 Å

EMDB-28711: CryoEM reconstruction of membrane-bound, left-handed CHMP1B+IST1 filament with 30% cholesterol
Method: EM (helical sym.) / Resolution: 4.4 Å

EMDB-28712: CryoEM reconstruction of membrane-bound, left-handed CHMP1B+IST1 filament with 40% cholesterol
Method: EM (helical sym.) / Resolution: 4.7 Å

EMDB-28713: CryoEM reconstruction of membrane-bound, left-handed CHMP1B(F9A/F13A)+IST1 filament with brominated SDPC probe
Method: EM (helical sym.) / Resolution: 2.9 Å

EMDB-28714: CryoEM reconstruction of membrane-bound, right-handed CHMP1B(F9A/F133A)+IST1 filament with brominated SDPC probe
Method: EM (helical sym.) / Resolution: 3.0 Å

EMDB-28715: CryoEM reconstruction of membrane-bound, left-handed CHMP1B(F9E/F13E)+IST1 filament with brominated SDPC probe
Method: EM (helical sym.) / Resolution: 3.4 Å

EMDB-28716: CryoEM reconstruction of membrane-bound, right-handed CHMP1B(F9E/F13E)+IST1 filament with brominated SDPC probe
Method: EM (helical sym.) / Resolution: 3.5 Å

EMDB-28717: CryoEM reconstruction of membrane-bound, left-handed CHMP1B(F9L/F13L)+IST1 filament with brominated SDPC probe
Method: EM (helical sym.) / Resolution: 3.2 Å

EMDB-28718: CryoEM reconstruction of membrane-bound, right-handed CHMP1B(F9L/F13L)+IST1 filament with brominated SDPC probe
Method: EM (helical sym.) / Resolution: 2.9 Å

EMDB-28719: CryoEM reconstruction of membrane-bound, right-handed, 34-subunit-per-turn CHMP1B+IST1 filament with POPC substituted for SDPC
Method: EM (helical sym.) / Resolution: 9.5 Å

EMDB-28722: CryoEM reconstruction of membrane-bound, right-handed CHMP1B+IST1 filament with brominated cholesterol probe
Method: EM (helical sym.) / Resolution: 4.3 Å

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  • Homo sapiens (human)

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