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TitleSynthesis, Biochemical Characterization, and Genetic Encoding of a 1,2,4-Triazole Amino Acid as an Acetyllysine Mimic for Bromodomains of the BET Family.
Journal, issue, pagesAngew. Chem. Int. Ed. Engl., Vol. 62, Page e202215460-e202215460, Year 2023
Publish dateSep 22, 2022 (structure data deposition date)
AuthorsKirchgassner, S. / Braun, M.B. / Bartlick, N. / Koc, C. / Reinkemeier, C.D. / Lemke, E.A. / Stehle, T. / Schwarzer, D.
External linksAngew. Chem. Int. Ed. Engl. / PubMed:36585954
MethodsX-ray diffraction
Resolution1.58 - 1.92 Å
Structure data

PDB-8b5a:
Human BRD3 bromodomain 2 in complex with a H4 peptide containing ApmTri (H4K20ApmTri)
Method: X-RAY DIFFRACTION / Resolution: 1.92 Å

PDB-8b5b:
Human BRD4 bromdomain 1 in complex with a H4 peptide containing acetyl lysine and ApmTri (H4K5acK8ApmTri)
Method: X-RAY DIFFRACTION / Resolution: 1.92 Å

PDB-8b5c:
Human BRD4 bromdomain 1 in complex with a H4 peptide containing ApmTri (H4K5/8ApmTri)
Method: X-RAY DIFFRACTION / Resolution: 1.58 Å

Chemicals

ChemComp-HOH:
WATER

ChemComp-PEG:
DI(HYDROXYETHYL)ETHER

Source
  • homo sapiens (human)
KeywordsPEPTIDE BINDING PROTEIN / ApmTri / acetyl-lysine mimic / BET-Bromodomain / histone-peptide

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