EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Two Distinct Modes of DNA Binding by an MCM Helicase Enable DNA Translocation.
ジャーナル・号・ページ	Int J Mol Sci, Vol. 23, Issue 23, Year 2022
掲載日	2022年11月24日
著者	Martin Meagher / Alexander Myasnikov / Eric J Enemark /
PubMed 要旨	A six-subunit ATPase ring forms the central hub of the replication forks in all domains of life. This ring performs a helicase function to separate the two complementary DNA strands to be replicated ...A six-subunit ATPase ring forms the central hub of the replication forks in all domains of life. This ring performs a helicase function to separate the two complementary DNA strands to be replicated and drives the replication machinery along the DNA. Disruption of this helicase/ATPase ring is associated with genetic instability and diseases such as cancer. The helicase/ATPase rings of eukaryotes and archaea consist of six minichromosome maintenance (MCM) proteins. Prior structural studies have shown that MCM rings bind one encircled strand of DNA in a spiral staircase, suggesting that the ring pulls this strand of DNA through its central pore in a hand-over-hand mechanism where the subunit at the bottom of the staircase dissociates from DNA and re-binds DNA one step above the staircase. With high-resolution cryo-EM, we show that the MCM ring of the archaeal organism binds an encircled DNA strand in two different modes with different numbers of subunits engaged to DNA, illustrating a plausible mechanism for the alternating steps of DNA dissociation and re-association that occur during DNA translocation.
リンク	Int J Mol Sci / PubMed:36499022 / PubMed Central
手法	EM (単粒子)
解像度	2.34 - 3.01 Å
構造データ	27974 8eaf EMDB-27974, PDB-8eaf: SsoMCM hexamer bound to Mg/ADP-BeFx and 12-mer oligo-dT. Class 1 手法: EM (単粒子) / 解像度: 2.62 Å 27975 8eag EMDB-27975, PDB-8eag: SsoMCM hexamer bound to Mg/ADP-BeFx and 12-mer oligo-dT. Class 2 手法: EM (単粒子) / 解像度: 3.01 Å 27976 8eah EMDB-27976, PDB-8eah: SsoMCM hexamer bound to Mg/ADP-BeFx and 16-mer oligo-dT. Class 1 手法: EM (単粒子) / 解像度: 2.48 Å 27977 8eai EMDB-27977, PDB-8eai: SsoMCM hexamer bound to Mg/ADP-BeFx and 16-mer oligo-dT. Class 2 手法: EM (単粒子) / 解像度: 2.76 Å 27978 8eaj EMDB-27978, PDB-8eaj: SsoMCM hexamer bound to Mg/ADP-BeFx and 46-mer DNA strand. Class 1 手法: EM (単粒子) / 解像度: 2.45 Å 27979 8eak EMDB-27979, PDB-8eak: SsoMCM hexamer bound to Mg/ADP-BeFx and 46-mer DNA strand. Class 2 手法: EM (単粒子) / 解像度: 2.67 Å 27980 8eal EMDB-27980, PDB-8eal: SsoMCM hexamer bound to Mg/ADP-BeFx and DNA. Class 1. Merged particles from datasets with 3 different DNA entities 手法: EM (単粒子) / 解像度: 2.34 Å 27981 8eam EMDB-27981, PDB-8eam: SsoMCM hexamer bound to Mg/ADP-BeFx and DNA. Class 2. Merged particles from datasets with 3 different DNA entities 手法: EM (単粒子) / 解像度: 2.59 Å
化合物	ChemComp-ZN: Unknown entry ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-08T: [[[(2R,3S,4R,5R)-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-tris(fluoranyl)beryllium ChemComp-HOH: WATER
由来	saccharolobus solfataricus p2 (古細菌) synthetic construct (人工物)
キーワード	REPLICATION / TRANSFERASE/DNA / Helicase / ATPase / TRANSFERASE-DNA complex