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TitleInsertions and deletions mediated functional divergence of Rossmann fold enzymes.
Journal, issue, pagesProc. Natl. Acad. Sci. USA, Vol. 119, Page e2207965119-e2207965119, Year 2022
Publish dateMay 8, 2022 (structure data deposition date)
AuthorsToledo-Patino, S. / Pascarelli, S. / Uechi, G.I. / Laurino, P.
External linksProc. Natl. Acad. Sci. USA / PubMed:36417431
MethodsX-ray diffraction
Resolution1.98 - 2.71 Å
Structure data

PDB-7xqm:
InDel-mutant short chain Dehydrogenase bound to SAH
Method: X-RAY DIFFRACTION / Resolution: 2.71 Å

PDB-7xqn:
InDel-mutant malate dehydrogenase from E. coli
Method: X-RAY DIFFRACTION / Resolution: 1.98 Å

Chemicals

ChemComp-SAH:
S-ADENOSYL-L-HOMOCYSTEINE

ChemComp-HOH:
WATER

Source
  • thermus thermophilus hb27 (bacteria)
  • escherichia coli (E. coli)
KeywordsBIOSYNTHETIC PROTEIN / Rossmann / mutant / InDel design / coenzyme-switch / oxidoreductase mutant / Rossmann divergence / modified coenzyme-binding

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